Variation in genomic Alu repeat density as a basis for rapid construction of low resolution physical maps of human chromosomes.
Variation in genomic Alu repeat density as a basis for rapid construction of low resolution physical maps of human chromosomes.
复制标题
基因组 Alu 重复密度的变化作为快速构建人类染色体低分辨率物理图谱的基础。
DOI：
10.1007/bf00346014
复制
发表时间：
1992
期刊：
影响因子：
1.6
通讯作者：
Hanlon,DJ
中科院分区：
文献类型：
--
作者：
Lane,MJ;Waterbury,PG;Carroll,WT;Smardon,AM;Faldasz,BD;Peshick,SM;Mante,S;Huckaby,CS;Kouri,RE;Hanlon,DJ
含有大量Alu重复序列的人DNA限制片段可以在人类DNA中存在其他人类DNA限制片段的情况下优先检测到：啮齿动物体细胞杂交时，DNA被罕见地用切割哺乳动物DNA的酶裂解。这种降低观察到的人类DNA复杂性的能力使我们能够开发一种方法来快速排序保留重叠的人类基因组结构域的体细胞杂交细胞系。排序过程还会生成Alu探针DNA检测到的人类片段的相对物理图谱。这一过程可以为整个染色体(100000 Kb)的人类基因组结构域生成物理图谱信息。这一策略是通过在一组老鼠中对Alu检测到的Noti片段进行排序来展示的：人类杂交细胞跨越了人类17号染色体的整个长臂。
Human DNA restriction fragments containing high numbers of Alu repeat sequences can be preferentially detected in the presence of other human DNA restriction fragments in DNA from human:rodent somatic cell hybrids when the DNA is fragmented with enzymes that cleave mammalian DNA infrequently. This ability to lower the observed human DNA complexity allowed us to develop an approach to order rapidly somatic hybrid cell lines retaining overlapping human genomic domains. The ordering process also generates a relative physical map of the human fragments detected with Alu probe DNA. This process can generate physical mapping information for human genomic domains as large as an entire chromosome (100,000 kb). The strategy is demonstrated by ordering Alu-detected NotI fragments in a panel of mouse:human hybrid cells that span the entire long arm of human chromosome 17.