Poly(tertiary amide acrylate) Copolymers Inspired by Poly(2-oxazoline)s: Their Blood Compatibility and Hydration States
Poly(tertiary amide acrylate) Copolymers Inspired by Poly(2-oxazoline)s: Their Blood Compatibility and Hydration States
复制标题
受聚（2-恶唑啉）启发的聚（叔酰胺丙烯酸酯）共聚物：它们的血液相容性和水合状态
DOI：
10.1021/acs.biomac.1c00411
复制
发表时间：
2021
影响因子：
6.2
通讯作者：
Tanaka Masaru
中科院分区：
文献类型：
--
作者：
Liu Shichen;Kobayashi Shingo;Sonoda Toshiki;Tanaka Masaru
用源自生物相容性聚合物的部分修饰聚(甲基)丙烯酸酯的侧链可以是开发新型血液相容性聚合物的有效方法。受生物相容性聚（2-甲基-2-恶唑啉）（PMeOx）和聚（2-乙基-2-恶唑啉）（PEtOx）的启发，合成了四种带有叔酰胺侧链的水溶性聚（叔酰胺酰化物）类似物。溶血和细胞活力测试结果表明，所有聚(叔酰胺酰化物)类似物均与红细胞、HeLa细胞和正常人真皮成纤维细胞相容，如PMeOx或PEtOx。在四种聚(叔酰胺酰化物)类似物中，聚[2-(N-甲基丙酰胺基)丙烯酸乙酯](PPEA)和聚[2-(N-乙基乙酰胺基)丙烯酸乙酯](PEAE)在水溶液中表现出热敏性；特别是，PPEA (10 mg mL–1) 表现出较低的临界溶液温度 37 °C。制备了水不溶性共聚物，以研究将这些合成聚合物用作血液相容性涂层的可能性。聚[甲基丙烯酸正丁酯70-co-2-(N-甲基乙酰胺基)乙基甲基丙烯酸酯30] (coPAEM)涂层显着抑制血浆蛋白吸附、吸附纤维蛋白原的变性程度和血小板粘附。通过差示扫描量热法在所有水合均聚物和共聚物中发现了中间水（IW），其含量通常可以表明聚合物的血液相容性。目前的工作表明，聚（甲基）丙烯酸酯侧链中的叔酰胺部分对血液相容性和 IW 具有有效的促进作用。
Modifying the side chain of poly(meth)acrylate with moieties originating from biocompatible polymers can be an effective method for developing novel blood-compatible polymers. Inspired by biocompatible poly(2-methyl-2-oxazoline) (PMeOx) and poly(2-ethyl-2-oxazoline) (PEtOx), four water-soluble poly(tertiary amide acylate) analogues bearing a pendant tertiary amide were synthesized. The results of hemolysis and cell viability tests showed that all the poly(tertiary amide acylate) analogues were compatible with red blood cells, HeLa cells, and normal human dermal fibroblasts as PMeOx or PEtOx. Among the four poly(tertiary amide acylate) analogues, poly[2-(N-methylpropionamido)ethyl acrylate] (PPEA) and poly[2-(N-ethylacetamido)ethyl acrylate] (PEAE) showed thermosensitivity in aqueous solution; especially, PPEA (10 mg mL–1) exhibited a lower critical solution temperature of 37 °C. Water-insoluble copolymers were prepared to investigate the possibility of applying these synthesized polymers as blood-compatible coatings. The poly[n-butyl methacrylate70-co-2-(N-methylacetamido)ethyl methacrylate30] (coPAEM) coatings significantly suppressed plasma protein adsorption, denaturation degree of adsorbed fibrinogen, and platelet adhesion. Intermediate water (IW), whose content can generally indicate the blood compatibility of polymers, was found in all hydrated homopolymers and copolymers by differential scanning calorimetry. The present work demonstrated that the tertiary amide moiety in the side chain of poly(meth)acrylate was an effective contributor to blood compatibility and IW.