NaCl-induced changes in cytosolic free Ca2+ in Arabidopsis thaliana are heterogeneous and modified by external ionic composition.
NaCl-induced changes in cytosolic free Ca2+ in Arabidopsis thaliana are heterogeneous and modified by external ionic composition.
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DOI：
10.1111/j.1365-3040.2008.01817.x
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发表时间：
2008-08
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影响因子：
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通讯作者：
F. E. Tracy;Matthew Gilliham;A. Dodd;A. Webb;M. Tester
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文献类型：
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作者：
F. E. Tracy;Matthew Gilliham;A. Dodd;A. Webb;M. Tester
胞质游离Ca（2+）（[Ca（2+）]（cyt））的增加在许多应激激活的信号通路中是常见的，包括对盐水环境的响应。利用水母发光蛋白研究了NaCl诱导的拟南芥幼苗[Ca（2+）]（cyt）信号的性质。我们发现NaCl诱导的[Ca（2+）]（cyt）的增加是不均匀的，主要限于根部。NaCl的浓度和浸泡根的溶液的组成对NaCl诱导的[Ca（2+）]（cyt）增加的幅度和动力学具有深远的影响。外源K（+）浓度的改变引起[Ca（2+）]（cyt）增加的时间和空间模式的改变，为Na（+）诱导的Ca（2+）跨膜内流提供了证据。各种药物对NaCl诱导的[Ca（2+）]（cyt）增加的影响表明，NaCl可通过质膜和细胞内Ca（2+）渗透通道诱导Ca（2+）内流。使用光子计数成像的时空[Ca（2+）]（cyt）动力学分析揭示了[Ca（2+）]（cyt）信号的额外复杂性水平，其可能反映了NaCl诱导的单细胞变化的振荡性质。
Increases in cytosolic free Ca(2+) ([Ca(2+)](cyt)) are common to many stress-activated signalling pathways, including the response to saline environments. We have investigated the nature of NaCl-induced [Ca(2+)](cyt) signals in whole Arabidopsis thaliana seedlings using aequorin. We found that NaCl-induced increases in [Ca(2+)](cyt) are heterogeneous and mainly restricted to the root. Both the concentration of NaCl and the composition of the solution bathing the root have profound effects on the magnitude and dynamics of NaCl-induced increases in [Ca(2+)](cyt). Alteration of external K(+) concentration caused changes in the temporal and spatial pattern of [Ca(2+)](cyt) increase, providing evidence for Na(+)-induced Ca(2+) influx across the plasma membrane. The effects of various pharmacological agents on NaCl-induced increases in [Ca(2+)](cyt) indicate that NaCl may induce influx of Ca(2+) through both plasma membrane and intracellular Ca(2+)-permeable channels. Analysis of spatiotemporal [Ca(2+)](cyt) dynamics using photon-counting imaging revealed additional levels of complexity in the [Ca(2+)](cyt) signal that may reflect the oscillatory nature of NaCl-induced changes in single cells.