[Relationship between M3 receptor and myocyte apoptosis induced by acute myocardial infarction].

[Relationship between M3 receptor and myocyte apoptosis induced by acute myocardial infarction].
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发表时间：
2004-05
期刊：
Yao xue xue bao = Acta pharmaceutica Sinica
影响因子：
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通讯作者：
Yan Liu;Yu-Hong Jing;Hong-li Sun;Hulun Li;Bao‐feng Yang
Yan Liu;Yu-Hong Jing;Hong-li Sun;Hulun Li;Bao‐feng Yang
中科院分区：
其他
文献类型：
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作者：
Yan Liu;Yu-Hong Jing;Hong-li Sun;Hulun Li;Bao‐feng Yang

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目的探讨M3受体在急性心肌梗死大鼠心肌细胞凋亡中的作用。方法采用结扎大鼠左冠状动脉前分支的方法建立心肌缺血模型。所有动物分为4组：假手术组、结扎组、胆碱组（10 mg × kg ~（-1））和4-二苯乙酰氧基-N-甲基哌啶-碘甲烷（4-diphenylacetoxy-N-methylpiperidine-methiodide，4DAMP）组（0.12 mg × kg ~（-1））。测定血清丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性。TTC染色确定心肌梗死面积。TUNEL法检测心肌细胞凋亡，免疫组化法检测心肌细胞凋亡相关蛋白Bcl-2、Fas的表达。结果M3受体激动剂胆碱能降低血清MDA含量，提高SOD活性。胆碱可使心肌Bcl-2表达增加，Fas表达减少。而阻断M_3受体则完全抑制胆碱对心肌细胞的上述作用。结论激活M3受体对急性心肌梗死后心肌细胞凋亡具有保护作用，其机制可能与调节Bcl-2、Fas等即早基因的表达有关。
AIM To explore the effects of M3 receptor on myocyte apoptosis induced by acute myocardial infarction in rats. METHODS Rat model was induced by ligation of the anterior branch of the left coronary artery. All animals were divided into four groups: sham-operated group, occlusion group, choline group (10 mg x kg(-1), iv), and 4DAMP (4-diphenylacetoxy-N-methylpiperidine-methiodide) group (0.12 mg x kg(-1), iv). The serum malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were determined. The infarct size areas on the myocardium were identified by TTC staining. The apoptosis in cardiomyocyte was detected by TUNEL assay and apoptosis-related proteins in Bcl-2 and Fas expression were measured by immunohistochemistry assay. RESULTS M3 receptor agonist choline reduced serum MDA content and increased SOD activity. The myocardial expression of Bcl-2 was increased, whereas the expression of Fas was decreased by choline. However, blockade of M3 receptor by 4DAMP completely inhibited these effects of choline on cardiac myocytes. CONCLUSION Activation of M3 receptor has protective effect on myocyte apoptosis induced by acute myocardial infarction in rat, and this effect might be related to modulating the expression of some immediateearly genes including Bcl-2 and Fas.