NATURE OF VIRALLY MEDIATED CHANGES IN MEMBRANE-PERMEABILITY TO SMALL MOLECULES
NATURE OF VIRALLY MEDIATED CHANGES IN MEMBRANE-PERMEABILITY TO SMALL MOLECULES
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DOI：
10.1042/bj1860847
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发表时间：
1980-01-01
影响因子：
4.1
通讯作者：
PASTERNAK, CA
中科院分区：
文献类型：
--
作者：
IMPRAIM, CC;FOSTER, KA;PASTERNAK, CA
进一步表征了仙台病毒引起的膜对小分子的通透性变化。集中在[小鼠癌Lettree]细胞内的物质的摄取被抑制。被磷酸化的胆碱和2-脱氧葡萄糖，以及由Na+连接机制驱动的氨基异丁酸盐和甘氨酸就是例子。在每一种化合物在质膜上的扩散受到速率限制的条件下，它的吸收是由病毒刺激的。例如胆碱，高浓度的2-脱氧葡萄糖和氨基酸，无Na+介质中的氨基酸和大多数低温物质。病毒介导的摄取减少显然是由于2个原因中的1个。由于磷酸化代谢物泄漏出细胞，磷酸化积累的物质不能保留。由于对Na+的渗透性增加导致梯度崩溃，通过连接到Na+梯度而积累的物质不再积累。对K+和Na+的渗透性增加导致膜去极化和细胞肿胀。后者可导致溶血（对红细胞而言），并可导致巨细胞（多核细胞）形成（对几种细胞类型而言）。细胞的恢复可以通过Ca2+的添加暂时实现；永久恢复需要在37度的温度下培养几个小时。C.讨论了病毒介导的通透性改变在临床情况和细胞生物学方面的可能意义。
The changes in membrane permeability to small molecules caused by Sendai virus were further characterized. The uptake of substances that are concentrated within [mouse carcinoma Lettree] cells is inhibited. Choline and 2-deoxyglucose, which become phosphorylated, and aminoisobutyrate and glycine, which are driven by a Na+-linked mechanism, are examples. The uptake of each compound under conditions where its diffusion across the plasma membrane is rate-limiting is stimulated by virus. Choline, 2-deoxyglucose and amino acids at high concentration, amino acids in Na+-free medium and most substances at low temperature are examples. Virally mediated decrease of uptake is apparently due to 1 of 2 causes. Substances that are accumulated by phosphorylation are not retained because of leakage of the phosphorylated metabolites out of cells. Substances that are accumulated by linkage to a Na+ gradient are no longer accumulated because of collapse of the gradient resulting from an increased permeability to Na+. Increased permeability to K+ and Na+ results in membrane depolarization and cell swelling. The latter event leads to hemolysis (for erythrocytes) and can lead to giant-cell (polykaryon) formation (for several cell types). Recovery of cells can be temporarily achieved by the addition of Ca2+; permanent recovery requires incubation for some hours at 37.degree. C. The possible significance of virally mediated permeability changes, with regard to clinical situations and to cell biology, is discussed.