Ultraviolet Radiation Exposure of One Eye Stimulates Sympathizing Expression of Neurokinin-1 Receptor but Not Monocyte Chemoattractant Protein-1 in the Partner Eye
Ultraviolet Radiation Exposure of One Eye Stimulates Sympathizing Expression of Neurokinin-1 Receptor but Not Monocyte Chemoattractant Protein-1 in the Partner Eye
复制标题
DOI：
10.1159/000501320
复制
发表时间：
2020-01-01
影响因子：
2.1
通讯作者：
Meyer, Linda M.
中科院分区：
文献类型：
--
作者：
Gross, Janine;Willimsky, Eva;Meyer, Linda M.
目的：探讨单侧紫外线照射（UVR）对未照射眼的影响。表征眼睛之间的免疫串扰，并通过P物质及其神经激肽-1受体（NKR-1）和/或单核细胞趋化蛋白-1（MCP-1）的神经激肽依赖性信号通路验证伴侣眼睛的交感反应。研究方法：利用紫外线照射生物光谱系统，将C57 BL/6小鼠单侧暴露于UVR-B中，使其白内障阈值等效剂量达到5倍，即14.5 kJ/m2。在照射期间，未暴露的对侧眼睛被完全屏蔽。暴露后3天和7天，用P物质NKR-1的荧光偶联抗体对眼睛进行染色。在仅接受麻醉但未接受UVR-B暴露的对照动物中进行相同的操作。通过酶联免疫吸附测定定量眼组织裂解物中的NKR-1和MCP-1水平。结果：UVR-B在暴露和未暴露的对侧小鼠眼中3天和7天后诱导NKR-1上调。暴露和对侧虹膜/睫状体复合体、脉络膜和对侧视网膜以及暴露角膜中的NKR-1蛋白水平上调。暴露的角膜、虹膜/睫状体复合体和房水中MCP-1水平升高，但对侧眼组织中没有。结论：UVR-B暴露不仅在暴露眼而且在未暴露眼的各种眼组织中触发NKR-1上调。在UVR-B暴露后，MCP-1蛋白水平在暴露的眼睛中上调，但对侧保持不受影响。(C)2019 S. Karger AG，巴塞尔。
Purpose: To investigate the influence of unilateral ultraviolet radiation (UVR) exposure on the unexposed, partner eye in vivo. To characterize the immunological cross-talk between the eyes and verify a sympathizing reaction of the partner eye via a neurokinin-dependent signaling pathway of substance P and its neurokinin-1 receptor (NKR-1) and/or monocyte chemoattractant protein-1 (MCP-1). Methods: C57BL/6 mice were unilaterally exposed in vivo to UVR-B to a 5-fold cataract threshold equivalent dose of 14.5 kJ/m(2) with a UV irradiation Bio-Spectra system. The unexposed contralateral eye was completely shielded during irradiation. After 3 and 7 days post exposure, eyes were stained with fluorescence-coupled antibody for substance P NKR-1. The same was performed in control animals receiving only anesthesia but no UVR-B exposure. NKR-1 and MCP-1 levels in ocular tissue lysates were quantified by enzyme-linked immunosorbent assay. Results: UVR-B induces NKR-1 upregulation after 3 and 7 days in the exposed and in the unexposed, contralateral mouse eye. NKR-1 protein level was upregulated in the exposed and contralateral iris/ciliary body complex, choroidea and in the contralateral retina as well as in the exposed cornea. MCP-1 levels were elevated in the exposed cornea, iris/ciliary body complex, and aqueous humor but not in contralateral ocular tissues. Conclusions: UVR-B exposure triggers NKR-1 upregulation not only in the exposed but also in the unexposed, partner eye in various ocular tissues. Following UVR-B exposure, MCP-1 protein levels are upregulated in the exposed eye, but the contralateral side remains unaffected. (C) 2019 S. Karger AG, Basel.