Similar rates of production of T and B lymphocytes in the bone marrow.
Similar rates of production of T and B lymphocytes in the bone marrow.
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DOI：
10.1084/jem.181.6.2201
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发表时间：
1995-06-01
期刊：
影响因子：
--
通讯作者：
Strober S
中科院分区：
文献类型：
--
作者：
Dejbakhsh-Jones S;Okazaki H;Strober S
通过用溴脱氧尿苷进行体内标记来估计胸腺正常的 C57BL/Ka 和无胸腺的 nu/nu BALB/c 小鼠骨髓中 T 淋巴细胞的更新率。根据 Thy-1、CD3 和 α/β T 细胞抗原受体标记的单克隆抗体的免疫荧光染色判断，T 淋巴细胞占正常小鼠骨髓细胞的 16-18%。大约 70% 的骨髓细胞表达骨髓细胞、巨噬细胞和 B 谱系细胞的受体（Mac-1、Gr-1、B220）。无胸腺骨髓中大约 13% 的细胞表达 α/β T 细胞受体。分选的骨髓 T 细胞在体外响应抗 α/β 抗体的刺激而增殖，并显示出 V β 和 J β 基因的功能重排。分选的非 T 细胞对体外刺激没有反应，并且所有鉴定出的 V β 和 J β 基因重排均无功能。体内标记研究表明，在胸腺正常的小鼠中，每天大约更新 17 x 10(6) 个骨髓 T 细胞，在无胸腺的小鼠中，每天更新大约 14 x 10(6) 个骨髓 T 细胞。后者小鼠的骨髓中每天大约更新 11 x 10(6) 个成熟 B 细胞（免疫球蛋白 M+）。为了确定骨髓前体是否可以直接产生T细胞，通过细胞分选去除胸腺正常和无胸腺小鼠的骨髓细胞中的T细胞，并在没有外源生长因子或胸腺基质细胞的情况下体外培养48小时。培养后对细胞的检查表明，10-12% 的 α/β T 细胞受体染色明亮。尽管在培养前未检测到Vβ和Jβ基因的功能重排，但大多数重排在培养后具有功能。培养物中明亮的 α/β T 细胞的出现取决于培养前骨髓细胞中 T 细胞的消耗。结果表明，大多数骨髓 T 细胞是在骨髓本身中产生的。
The rate of renewal of T lymphocytes in the bone marrow of euthymic C57BL/Ka and athymic nu/nu BALB/c mice was estimated by in vivo labeling with bromodeoxyuridine. T lymphocytes accounted for 16-18% of marrow cells in euthymic mice as judged by immunofluorescent staining with monoclonal antibodies for Thy-1, CD3, and alpha/beta T cell antigen receptor markers. About 70% of marrow cells expressed receptors (Mac-1, Gr-1, B220) for myeloid, macrophage, and B lineage cells. Approximately 13% of cells in the athymic bone marrow expressed alpha/beta T cell receptors. Sorted marrow T cells proliferated in response to stimulation with anti-alpha/beta antibodies in vitro and showed functional rearrangements of V beta and J beta genes. Sorted non- T cells did not respond to stimulation in vitro, and all V beta and J beta gene rearrangements identified were nonfunctional. In vivo labeling studies indicated that approximately 17 x 10(6) bone marrow T cells are renewed daily in euthymic mice and approximately 14 x 10(6) are renewed in athymic mice. Approximately 11 x 10(6) mature B cells (immunoglobulin M+) are renewed daily in the bone marrow of the latter mice. To determine whether marrow precursors can give rise to T cells directly, marrow cells from euthymic and athymic mice were depleted of T cells by cell sorting and incubated in vitro for 48 h in the absence of exogenous growth factors or thymic stromal cells. Examination of the cells after culture showed that 10-12% stained brightly for alpha/beta T cell receptors. Although functional rearrangements of V beta and J beta genes were not detected before culture, the majority of rearrangements were functional after culture. The emergence of the bright alpha/beta T cells in culture was dependent on depletion T cells from the marrow cells before culture. The results suggest that most marrow T cells are generated in the marrow itself.
影响因子：
15.3
作者：
MINATO, N;HATTORI, M;SUDO, T;KANO, S;MIURA, Y;SUDA, J;SUDA, T
通讯作者：
SUDA, T