The Mi-2/NuRD complex associates with pericentromeric heterochromatin during S phase in rapidly proliferating lymphoid cells
The Mi-2/NuRD complex associates with pericentromeric heterochromatin during S phase in rapidly proliferating lymphoid cells
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DOI：
10.1007/s00412-009-0207-7
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发表时间：
2009-08-01
期刊：
影响因子：
1.6
通讯作者：
Wade, Paul A.
中科院分区：
文献类型：
--
作者：
Chadwick, Lisa Helbling;Chadwick, Brian P.;Wade, Paul A.
染色体复制不仅导致DNA序列的复制，还导致组蛋白修饰、DNA甲基化和核蛋白结构的复制，这些都构成了表观遗传信息。人类细胞中的着丝粒周围异染色质的特征在于独特的组蛋白和DNA修饰模式。在这里，我们描述了协会的Mi-2/NuRD复合物与特定片段的着丝粒周围异染色质组成的卫星II/III DNA位于人类染色体1，9和16，在一些但不是所有的细胞类型。这种关联部分与DNA复制和染色质组装有关，并可能在这些过程中发挥作用。Mi-2/NuRD积累不依赖于Polycomb缔合，其特征在于组蛋白修饰的独特模式。我们提出Mi-2/NuRD构成了快速增殖的淋巴细胞用于复制组成型异染色质的染色质组装和成熟途径的酶组分。
Chromosomal replication results in the duplication not only of DNA sequence but also of the patterns of histone modification, DNA methylation, and nucleoprotein structure that constitute epigenetic information. Pericentromeric heterochromatin in human cells is characterized by unique patterns of histone and DNA modification. Here, we describe association of the Mi-2/NuRD complex with specific segments of pericentromeric heterochromatin consisting of Satellite II/III DNA located on human chromosomes 1, 9, and 16 in some but not all cell types. This association is linked in part to DNA replication and chromatin assembly and may suggest a role in these processes. Mi-2/NuRD accumulation is independent of Polycomb association and is characterized by a unique pattern of histone modification. We propose that Mi-2/NuRD constitutes an enzymatic component of a pathway for assembly and maturation of chromatin utilized by rapidly proliferating lymphoid cells for replication of constitutive heterochromatin.