Temperature-sensitive reaction intermediate of F1-ATPase.
Temperature-sensitive reaction intermediate of F1-ATPase.
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F1-ATPase的温度敏感反应中间体。
DOI：
10.1038/sj.embor.7401135
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发表时间：
2008-01
期刊：
影响因子：
7.7
通讯作者：
Noji, Hiroyuki
中科院分区：
文献类型：
--
作者：
Watanabe, Rikiya;Iino, Ryota;Shimabukuro, Katsuya;Yoshida, Masasuke;Noji, Hiroyuki
F1-ATPase 是一种旋转分子马达，可进行 120° 步进旋转，每一步均由单个 ATP 水解驱动。在这项研究中，在低于 4°C 的温度下发现了一种新的 F1-ATPase 反应中间体，该中间体在与 ATP 结合时以相同的角度旋转暂停。中间体反应的速率常数强烈依赖于温度，Q10 因子为 19，这意味着中间体反应伴随着较大的构象变化。动力学分析表明中间状态不对应于 ATP 结合或水解。向反应混合物中添加ADP并没有改变中间态的角位置，但特别延长了该态的时间常数。相反，添加无机磷酸盐导致在与中间状态+80°角处停顿。这些观察结果强烈表明新发现的反应中间体是 ADP 释放步骤。
F1-ATPase is a rotary molecular motor that makes 120° stepping rotations, with each step being driven by a single-ATP hydrolysis. In this study, a new reaction intermediate of F1-ATPase was discovered at a temperature below 4°C, which makes a pause at the same angle in its rotation as when ATP binds. The rate constant of the intermediate reaction was strongly dependent on temperature with a Q10 factor of 19, implying that the intermediate reaction accompanies a large conformational change. Kinetic analyses showed that the intermediate state does not correspond to ATP binding or hydrolysis. The addition of ADP to the reaction mixture did not alter the angular position of the intermediate state, but specifically lengthened the time constant of this state. Conversely, the addition of inorganic phosphate caused a pause at an angle of +80° from that of the intermediate state. These observations strongly suggest that the newly found reaction intermediate is an ADP-releasing step.
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影响因子：
16.8
作者：
Ariga, Takayuki;Muneyuki, Eiro;Yoshida, Masasuke
通讯作者：
Yoshida, Masasuke
DOI：
10.1073/pnas.0406486102
发表时间：
2005-03-22
影响因子：
11.1
作者：
Hirono-Hara, Y;Ishuzuka, K;Noji, H
通讯作者：
Noji, H
影响因子：
64.8
作者：
Yasuda, R;Noji, H;Itoh, H
通讯作者：
Itoh, H
影响因子：
3.4
作者：
Muneyuki, Eiro;Watanabe-Nakayama, Takahiro;Noji, Hiroyuki
通讯作者：
Noji, Hiroyuki
影响因子：
64.8
作者：
Sabbert, D;Engelbrecht, S;Junge, W
通讯作者：
Junge, W