Fine structure of the recB and recC gene region of Escherichia coli.
Fine structure of the recB and recC gene region of Escherichia coli.
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大肠杆菌recB和recC基因区域的精细结构。
DOI：
10.1016/0006-291x(82)91737-5
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发表时间：
1982
影响因子：
3.1
通讯作者：
Y. Takagi
中科院分区：
文献类型：
--
作者：
M. Sasaki;T. Fujiyoshi;K. Shimada;Y. Takagi
为了进行recBC DNase的遗传和酶学研究，将粘粒中克隆的大肠杆菌thyA、recC、recB和argA基因区亚克隆到pBR322质粒载体中，并制作其精细限制性图谱。互补分析表明，据估计，这些基因位于 19kb Bam HI 片段中，顺序为 thyA recC recB argA。含有用该 Bam HI 片段亚克隆的质粒的recA recB recC 细胞表现出recBC DNase 活性增加了12.3 倍。携带recB和recC基因的克隆分别表达recB和recC功能，通过S1核酸酶图谱检测到这些基因的转录起始位点，证明recB和recC基因由独立的顺反子组成。
For genetic and enzymatic study of recBC DNase, the Escherichia coli thyA, recC, recB and argA gene region cloned in a cosmid was subcloned into the pBR322 plasmid vector and its fine restriction map was made. Complementation analysis showed that these genes were located in a 19kb Bam HI fragment in the order thyA recC recB argA, so far as estimated. The recA recB recC cells harboring plasmid subcloned with this Bam HI fragment exhibited 12.3-fold increase in recBC DNase activity. Clones carrying the recB and the recC gene expressed recB and recC function, respectively, and the initiation sites of transcription of these genes were detected by S1 nuclease mapping, proving that the recB and recC genes consist of independent cistrons.