Phylogenetic placement of uncultured Ceanothus microsymbionts using 16S rRNA gene sequences
Phylogenetic placement of uncultured Ceanothus microsymbionts using 16S rRNA gene sequences
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DOI：
10.1139/b99-080
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发表时间：
1999-12
期刊：
影响因子：
1.1
通讯作者：
N. J. Ritchie;D. Myrold
中科院分区：
文献类型：
--
作者：
N. J. Ritchie;D. Myrold
从美洲Ceanothus americanus L.根瘤中直接扩增出16S rDNA全长序列。和三花Ceanothus thyrsiflorus P.使用聚合酶链式反应。使用自动测序仪测定序列，与GenBank中的序列进行比较，并组装成共有序列。将序列与数据库中可获得的其他全长Frankia 16S rDNA序列进行比对。系统发生树，使用三种不同的算法：邻居加入，简约，和最大似然法。三种方法均表明，这些Ceanothus L.微共生体与Dryasdrummondii Richardson ex Hook的微共生体关系最为密切。Lvs.而不是从胡颓子科分离的Frankia.关键词：Frankia，Ceanothus，16S rDNA.
Full-length 16S rDNA sequences were amplified directly from the nodules of Ceanothus americanus L. and Ceanothus thyrsiflorus Eschsch. using the polymerase chain reaction. Sequences were determined using an automated sequencer, compared against those in GenBank, and assembled into consensus sequences. The sequences were aligned with other full-length Frankia 16S rDNA sequences available from the data base. Phylogenetic trees were obtained using three different algorithms: neighbor joining, parsimony, and the maximum-likelihood method. All three methods showed that these Ceanothus L. microsymbionts were most closely related to the microsymbiont associated with Dryas drummondii Richardson ex Hook. Lvs. rather than Frankia isolated from the Elaeagnaceae.Key words: Frankia, Ceanothus, 16S rDNA.