Geographic differences in the sensitivity of a polymerase chain reaction for the detection of Plasmodium falciparum infection
Geographic differences in the sensitivity of a polymerase chain reaction for the detection of Plasmodium falciparum infection
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DOI：
10.4269/ajtmh.1996.55.647
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发表时间：
1996-12-01
影响因子：
3.3
通讯作者：
Kilian, AHD
中科院分区：
文献类型：
--
作者：
Jelinek, T;Proll, S;Kilian, AHD
利用聚合酶链反应（PCR）对目标DNA进行高度特异性的扩增，为检测疟疾感染提供了一种高度敏感和特异性的方法。在一个调查区域内具有不同地方性的环境中使用聚合酶链反应尚未进行深入调查。因此，在乌干达西部的Kabarole和Bundibugyo地区进行了一项横断面研究，使用了来自疟疾流行病学情况不同的三个村庄的材料和PCR的DNA引物，这些引物在以前的试验中显示出令人满意的敏感性和特异性。3个调查村的PCR敏感性差异显著（P < 0.001） （K1-14-1引物的敏感性在63.2% ~ 83.9%之间，MSP-1引物的敏感性在37.9% ~ 69.9%之间），且与该地区居民的地理差异和社会交流密切相关。根据调查结果，在流行病学现场研究中，不建议使用单一引物对检测恶性疟疾。建议使用组合引物对，并经常通过显微镜确认结果。
The amplification of target DNA by highly specific probes using the polymerase chain reaction (PCR) provides a highly sensitive and specific method for the detection of malaria infection. The use the of PCR in settings with varying endemicity within one survey area has not been investigated intensively. Therefore, a cross-sectional study was conducted in the districts of Kabarole and Bundibugyo in western Uganda using material from three villages with different epidemiologic situations regarding malaria and DNA primers for a PCR that had shown satisfactory sensitivity and specificity in previous trials. The sensitivity of the PCR varied significantly (P < 0.001) in the three survey Villages (between 63.2% and 83.9% for the primer pair K1-14-1 and between 37.9% and 69.9% for the primer pair MSP-1) and was highly linked to geographic differences and social exchanges of the inhabitants with other areas of the district. According to the results of this investigation, it is advisable not to use a single primer pair in epidemiologic field studies for the detection of falciparum malaria. The use of combined primer pairs and the frequent confirmation of the results by microscopy are recommended.