Healing Following Implantation Of Root With Remaining Periodontal Ligament Cultured In Vitro
Healing Following Implantation Of Root With Remaining Periodontal Ligament Cultured In Vitro
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DOI：
10.4172/2161-1122.1000234
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发表时间：
2014-04
期刊：
影响因子：
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通讯作者：
A. Saito;E. Saito
中科院分区：
文献类型：
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作者：
A. Saito;E. Saito
背景：意向再植是指拔出牙齿进行口外根管治疗，刮除根尖病变（如果存在），然后将其替换到牙槽中。有意再植牙齿失败的最常见原因是牙周膜损伤引起的外部吸收和强直。我们推测，如果来自健康牙周膜的增殖细胞能够覆盖受损部位，那么种植和再植的成功率就会提高，从而扩大其应用范围。本研究的目的是从组织学角度评估组织培养对将体外培养的牙根连同剩余牙周韧带植入下颌骨腔后愈合的影响。方法：拔除5只比格犬的20颗门牙，用车针修整40颗牙根（4.0×3.0 mm）。保留三十根修剪后的牙根的根面牙周膜，其余十根修剪后的牙根去除根面的牙周膜。将30根修剪后保留牙周膜的牙根按照培养顺序分为三组，即0周（不培养）、2周和4周。培养期结束后，将根植入下颌骨中形成的骨腔中。术后四个星期，制备标本用于组织学分析。结果：0w组10例中有3例出现强直。然而，2周和4周组中根本没有发生强直。然而，三组之间任何参数（正常牙周、强直、表面吸收、炎症吸收）均无显着差异。结论：拔牙过程中发生的机械损伤可能是造成牙齿强直的原因。如果整个牙根表面都覆盖有培养的牙周膜来源细胞，可以防止关节强直，则意向再植和种植的成功率可能会提高。
Background: Intentional replantation is extraction of a tooth to do extraoral root canal therapy, curettage of apical lesion when present, and its replacement in its socket. The most common causes of failure in intentionally replanted teeth are external resorption and ankylosis caused by periodontal ligament damage. We hypothesized that if proliferative cells derived from healthy periodontal ligament could cover a damaged site, the success rate of implantation and replantation would increase and thus widen its application. The purpose of this study was to histologically evaluate the influence of tissue culture on healing following implantation of root with remaining periodontal ligament cultured in vitro into a mandibular bone cavity. Methods: Twenty incisors of 5 beagles were extracted and forty trimmed roots (4.0×3.0 mm) were made using a bur. Periodontal ligament on the root surface of thirty trimmed roots was retained and remaining ten trimmed roots were removed periodontal ligament on the root surface. Thirty trimmed roots with remaining periodontal ligament were divided into three groups according to each culture sequence, namely 0 week (not cultured), 2 and 4 weeks. Following the culture period, the roots were implanted into bone cavities created in the mandible. Four weeks postsurgery, the specimens were prepared for histological analysis. Results: In the 0w group, ankylosis was observed in three of 10 cases. However, ankylosis did not occur at all in the 2w and 4w groups. However, there were no significant differences in any parameter (normal periodontum, ankylosis, surface resorption, inflammatory resorption) among the three groups. Conclusion: The mechanical injury that occurred during extraction of the teeth could be responsible for the ankylosis. If the entire root surface is covered with cultured periodontal ligament-derived cells and that could prevent ankylosis, the success rate of intentional replantation and implantation might increase.