In vitro study of soil arsenic release by human gut microbiota and its intestinal absorption by Caco-2 cells.
In vitro study of soil arsenic release by human gut microbiota and its intestinal absorption by Caco-2 cells.
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DOI：
10.1016/j.chemosphere.2016.10.091
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发表时间：
2017-02
期刊：
影响因子：
8.8
通讯作者：
Naiyi Yin;Xiaolin Cai;H. Du;Zhennan Zhang;Zejiao Li;Xiaochen Chen;Guoxin Sun;Yanshan Cui
中科院分区：
文献类型：
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作者：
Naiyi Yin;Xiaolin Cai;H. Du;Zhennan Zhang;Zejiao Li;Xiaochen Chen;Guoxin Sun;Yanshan Cui
砷（As）的形态分析是评价砷污染土壤健康风险的基础。评价了土壤结合砷的释放、人体肠道菌群对砷的转化以及土壤砷代谢产物的后续肠道吸收。培养动态人肠道模型中的结肠微生物群落和肠上皮细胞系Caco-2。砷形态分析和吸收不同形态的砷进行。在这项研究中，在结肠中观察到土壤As释放（3.7-581.2 mg kg−1）。砷在大肠杆菌中的转化速度比在土壤固相中快。X射线吸收近边光谱（XANES）分析表明，由于砷酸盐[As（V）]还原而产生的44.2-97.6%的亚砷酸盐[As（III）]在结肠相之后的土壤固相中。土壤As代谢产物的细胞吸收率较高，单甲基胂酸和As（III）的肠吸收率分别为33.6%和30.2%。对砷的富集率略高于二甲基胂酸和As（V）（分别为25.1%和21.7%）。我们的研究结果表明，人类肠道微生物群可以直接释放土壤结合砷，特别是含砷的无定形铁/铝氧化物。同时测定砷的转化和肠道吸收，将导致准确的风险评估与土壤砷暴露的人体健康。
Arsenic (As) speciation is essential in assessing health risks from As-contaminated soil. Release of soil-bound arsenic, As transformation by human gut microbiota, and the subsequent intestinal absorption of soil As metabolites were evaluated. A colon microbial community in a dynamic human gut model and the intestinal epithelial cell line Caco-2 were cultured. Arsenic speciation analysis and absorption of different As species were undertaken. In this study, soil As release (3.7–581.2 mg kg−1) was observed in the colon. Arsenic in the colon digests was transformed more quickly than that in the soil solid phase. X-ray absorption near-edge spectroscopy (XANES) analysis showed that 44.2–97.6% of arsenite [As(III)] generated due to arsenate [As(V)] reduction was in the soil solid phase after the colon phase. We observed a high degree of cellular absorption of soil As metabolites, exhibiting that the intestinal absorption of monomethylarsonic acid and As(III) (33.6% and 30.2% resp.) was slightly higher than that of dimethylarsinic acid and As(V) (25.1% and 21.7% resp.). Our findings demonstrate that human gut microbiota can directly release soil-bound arsenic, particularly As-bearing amorphous Fe/Al-oxides. Determining As transformation and intestinal absorption simultaneously will result in an accurate risk assessment of human health with soil As exposures.