Amphiphilic Co-Solvents Modulate the Structure of Membrane Domains
Amphiphilic Co-Solvents Modulate the Structure of Membrane Domains
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DOI：
10.1021/acssuschemeng.2c06876
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发表时间：
2023-01
期刊：
影响因子：
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通讯作者：
Luoxi Tan;H. L. Scott;M. Smith;S. Pingali;H. O’Neill;J. Morrell-Falvey;J. Katsaras;Jeremy C. Smith-Jer
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文献类型：
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作者：
Luoxi Tan;H. L. Scott;M. Smith;S. Pingali;H. O’Neill;J. Morrell-Falvey;J. Katsaras;Jeremy C. Smith-Jer
生物燃料是可持续能源的一个日益重要的组成部分。这使得优化生物燃料生产成为社会和经济的当务之急。减轻两亲性共溶剂的毒性作用是提高生物燃料生产效率的一种方法。两亲物进入细胞膜，导致膜变薄、不稳定、膜电位丧失，并最终导致细胞死亡。然而，这种溶剂毒性的图片错过了共溶剂对侧向膜组织的破坏性影响，这越来越被认为是膜蛋白分选和寡聚化的关键。膜结构域的改变或破坏对细胞过程具有有害影响。在这项工作中，我们追求的假设，即膜的横向组织被破坏的存在下，浓度低于那些导致完全膜不稳定的共溶剂。由于共存相之间的界面张力增加而发生破坏，导致构象变化以使界面长度与面积比最小化。这代表了一种未被认识到的溶剂诱导应激模式和提高发酵产率的干预新目标。
Biofuels are an increasing part of the sustainable energy picture. This makes it a societal and economic imperative to optimize biofuel production. Mitigating the toxic effects of amphiphilic co-solvents is one way to improve the efficiency of biofuel production. Amphiphiles partition into cellular membranes, leading to membrane thinning, destabilization, loss of membrane potential, and, ultimately, cell death. However, this picture of solvent toxicity misses the disruptive impact of co-solvents on lateral membrane organization, which is increasingly recognized as critical for membrane protein sorting and oligomerization. The alteration or disruption of membrane domains has deleterious effects on cellular processes. In this work, we pursue the hypothesis that membrane lateral organization is disrupted by the presence of co-solvents at concentrations lower than those which lead to full membrane destabilization. The disruption occurs due to an increasing interfacial tension between the co-existing phases, resulting in conformational changes to minimize the interfacial length-to-area ratio. This represents an unrecognized mode of solvent-induced stress and a new target for interventions to improve fermentation yields.