Growth of spermatogenetic colonies in the mouse testis after irradiation with fission neutrons.
Growth of spermatogenetic colonies in the mouse testis after irradiation with fission neutrons.
复制标题
裂变中子照射后小鼠睾丸中生精集落的生长。
DOI：
10.2307/3575692
复制
发表时间：
1982
影响因子：
3.4
通讯作者：
J. Davids
中科院分区：
文献类型：
--
作者：
G. J. van den Aardweg;A. L. de Ruiter;M. Kramer;J. Davids
照射后，CBA小鼠睾丸中的抗辐射干细胞产生生精细胞集落，这些集落通过纵向生长重新填充小管。在100和300拉德中子剂量后3至20周，通过（1）直接测量集落长度，（2）体视学测量集落长度，（3）使用再增殖指数（RI）方法（A.L. de Ruiter-Bootsma，M.F.克雷默de Rooij和J.A.G.大卫，在中子辐照的生物效应，pp。325-334，IAEA，维也纳，1974年; Radiat. Res. 67，56-68，（1976））。直接法表明，300拉德照射后的菌落数从4周开始稳定，在此剂量后，4周时发现单个菌落长度有很大变化，15周时变化更大。一旦形成，菌落显然具有差异很大的内在生长速率。根据体视学测量，可以计算总的再生管长度（L/sub r/）。在100拉德中子照射后的前6周，L/sub r/的增加被由电池耗尽引起的管收缩超过，导致恒定的L/sub r/。一旦细胞耗尽完成，L/sub/r/以6.7的生长速率线性增加。Mu.. m/菌落/天，从6周时占总小管长度的50%增加到20周时的80%。在300拉德的中子剂量后，细胞消耗在3周内完成，因此菌落的生长直接反映在L/sub r/的增加中。殖民地的生长在8周内呈线性增长，增长率为33. Mu.. m/菌落/天。尽管存在少量的菌落，但它们的生长在较长的照射后时间内逐渐减慢。直接法和体视学法的结果在L/sub r/上吻合得很好。«少
After irradiation, radioresistant stem cells in CBA mouse testes give rise to colonies of spermatogenetic cells, which repopulate the tubules by longitudinal outgrowth. The mode of growth was followed in histological testes sections from 3 to 20 weeks after doses of 100 and 300 rad neutrons by measuring the length of the colonies (1) directly, (2) stereologically, and (3) with use of the repopulation index (RI) method (A.L. de Ruiter-Bootsma, M.F. Kramer, D.G. de Rooij, and J.A.G. Davids, in Biological Effects of Neutron Irradiation, pp. 325-334, IAEA, Vienna, 1974; Radiat. Res. 67, 56-68, (1976)). The direct method showed that the number of colonies present after 300 rad is stable from 4 weeks on. After this dose great variation in individual colony length was found at 4 weeks, and it was even greater at 15 weeks. Once formed, the colonies apparently have intrinsic growth rates which differ widely. From the stereological measurements a total repopulating tubular length (L/sub r/) could be calculated. In the first 6 weeks after 100 rad neutrons the increase in L/sub r/ is exceeded by tubular shrinkage, caused by cell depletion, resulting in a constant L/sub r/. Once cell depletion has finished, L/sub r/ increases linearly withmore » a growth rate of 6.7 ..mu..m/colony/day from 50% of the total tubular length at 6 weeks to 80% at 20 weeks. After a dose of 300 rad neutrons cell depletion finishes within 3 weeks and hence the growth of the colonies is directly reflected in an increase of L/sub r/. The colony growth is linear up to 8 weeks with a rate of 33 ..mu..m/colony/day. Despite the low number of colonies present, their growth gradually slows over longer postirradiation times. The results of the direct and stereological methods agree very well on L/sub r/.« less