Association of cadmium and arsenic exposure with salivary telomere length in adolescents in Terai, Nepal
Association of cadmium and arsenic exposure with salivary telomere length in adolescents in Terai, Nepal
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DOI：
10.1016/j.envres.2016.04.037
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发表时间：
2016-08-01
影响因子：
8.3
通讯作者：
Watanabe, Chiho
中科院分区：
文献类型：
--
作者：
Fillman, Toki;Shimizu-Furusawa, Hana;Watanabe, Chiho
背景：镉和砷是环境中普遍存在的危害人类健康的金属。越来越多的研究表明，端粒长度是未来疾病风险的潜在生物标记物。很少有研究研究金属对端粒长度的影响，也没有研究集中在青少年身上。目的：本研究研究了镉和砷对尼泊尔特莱青少年唾液端粒长度的影响。方法：对351名12-16岁的青少年进行问卷调查和唾液和尿液采集。用从唾液中提取的DNA进行定量聚合酶链式反应测定端粒长度。电感耦合等离子体质谱检测尿镉、砷浓度。结果：镉、砷的几何均值和标准差分别为0.33+/-0.33mU/g肌酐和196.0/-301.1 mU/g肌酐。调整混杂因素后，尿镉浓度与唾液端粒长度呈负相关(β=-0.24，95%CI-0.42，-0.07)。砷与端粒长度呈正相关，但没有达到统计学意义。结论：这是首次研究证明镉可能会缩短青少年的端粒，即使在可能被认为是低水平的暴露水平。这些结果与先前的实验和成人流行病学研究一致，也有助于确定镉损伤DNA的机制。这项研究扩大了目前关于镉暴露对端粒长度的有害影响的证据，甚至对青少年也是如此。(C)2016 Elsevier Inc.保留所有权利。
Background: Cadmium and arsenic are ubiquitous metals commonly found in the environment which can harm human health. A growing body of research shows telomere length as a potential biomarker of future disease risk. Few studies have examined the effects of metals on telomere length and none have focused on adolescents.Objectives: In this study, the impact of cadmium and arsenic on salivary telomere length was studied in adolescents in Terai, Nepal.Methods: Adolescents aged 12-16 years old (n=351)were recruited where questionnaire interviews and both saliva and urine collection took place. Telomere length was determined by quantitative polymerase chain reaction using DNA extracted from saliva. Urinary cadmium and arsenic concentration were measured by inductively coupled plasma mass spectrometry. Multivariable linear regression was used to examine associations between urinary metals and salivary telomere length.Results: The geometric means and standard deviations of cadmium and arsenic were 0.33 +/- 0.33 mu g/g creatinine and 196.0 +/- 301.1 mu g/g creatinine, respectively. Urinary cadmium concentration was negatively associated with salivary telomere length after adjustment for confounders (beta=-0.24, 95% CI -0.42, -0.07). Arsenic showed positive associations with telomere length but did not reach statistical significance.Conclusions: This is the first study to demonstrate that cadmium may shorten adolescent telomeres, even at exposure levels that may be considered low. These results agree with prior experimental and adult epidemiological studies, and also help identify the mechanism of DNA damage by cadmium. This study expanded current evidence on the harmful effects of cadmium exposure on telomere length even to adolescents. (c) 2016 Elsevier Inc. All rights reserved.