Cells expressing the human foamy virus (HFV) accessory Bet protein are resistant to productive HFV superinfection
Cells expressing the human foamy virus (HFV) accessory Bet protein are resistant to productive HFV superinfection
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DOI：
10.1006/viro.1998.9362
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发表时间：
1998-10-10
期刊：
影响因子：
3.7
通讯作者：
Rethwilm, A
中科院分区：
文献类型：
--
作者：
Bock, M;Heinkelein, M;Rethwilm, A
Bet是一种泡沫病毒（FV）辅助蛋白，不是病毒复制所必需的。Bet的功能还不清楚。我们报告了稳定表达HFV Bet蛋白的细胞系的产生。在Bet（+）细胞中，HFV复制与对照细胞相比降低了约3-4个数量级。HFV Bet表达细胞仅部分抵抗远亲猫FV（FFV）的感染。假型实验，使用鼠逆转录病毒载体与HFV包膜，揭示了耐药性不是由于未知HFV受体的下调。在转染实验中，使用前病毒报告基因构建体和感染性前病毒，在Bet（+）和对照细胞之间没有检测到显著差异。在感染实验中，在HFV启动子控制下表达指示基因的HFV载体在Bet（+）细胞中显示无活性。结果是最好的兼容的假设，生产性的Bet+细胞的超感染的主要块发生在病毒进入和前病毒建立之间的复制的早期阶段。我们认为，抑制前病毒整合的Bet蛋白可能是一个独特的功能，在独特的泡沫病毒复制周期。(C)北京：科学出版社.
Bet is a foamy virus (FV) accessory protein not required for virus replication. The function of Bet is not understood. We report on the generation of cell lines stably expressing the HFV Bet protein. In Bet(+) cells, HFV replication was reduced by similar to 3-4 orders of magnitude compared with control cells. The HFV Bet-expressing cells only partially resisted infection by the distantly related feline FV (FFV). Pseudotyping experiments, using murine retroviral vectors with an HFV envelope, revealed that the resistance was not due to downregulation of the unknown HFV receptor. In transfection experiments, using proviral reporter gene constructs and infectious proviruses, no significant differences were detected between Bet(+) and control cells. In infection experiments, HFV vectors expressing an indicator gene under control of the HFV promoters showed no activity in Bet(+) cells. The results are best compatible with the hypothesis that the main block to productive superinfection of Bet+ cells occurs at an early stage of replication between virus entry and provirus establishment. We suggest that inhibition of provirus integration by Bet protein may serve a distinct function in the unique foamy virus replication cycle. (C) 1998 Academic Press.