Enhanced antimicrobial activity of silver nanoparticles conjugated with synthetic peptide by click chemistry
Enhanced antimicrobial activity of silver nanoparticles conjugated with synthetic peptide by click chemistry
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DOI：
10.1007/s11051-020-04799-6
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发表时间：
2020-04-15
影响因子：
2.5
通讯作者：
Rodriguez-Reyes, Juan Carlos F.
中科院分区：
文献类型：
--
作者：
Gakiya-Teruya, Miguel;Palomino-Marcelo, Luis;Rodriguez-Reyes, Juan Carlos F.
设计新型抗菌材料的策略可能依赖于材料的组合以实现协同效应。然而，抗菌肽与纳米颗粒的偶联需要方便地指导以避免肽内的结构变化和/或纳米颗粒的降解。在这里，我们提出了一个合成的肽（VIHGW-炔-G-NH 2）含有氨基末端铜和镍（ATCUN）基序的银纳米粒子的附件的结果。为了指导肽-纳米颗粒偶联，用炔官能化肽，而使用硫醇-聚乙二醇-叠氮化物（HS-PEG-N-3）链用叠氮基官能化纳米颗粒，使得乙炔化物和叠氮化物可以经历点击反应。该反应在室温下进行，并且在构建纳米颗粒-PEG-ATCUN阵列的步骤之后进行UV-Vis吸收光谱、X射线光电子能谱（XPS）和红外光谱的组合。PEG分子通过硫醇末端连接的证据表明纳米颗粒用叠氮基官能化，尽管仅部分官能化。与合成肽的点击反应通过用红外光谱法损失N-3-振动信号来证明。在整个合成步骤中，通过紫外-可见光谱、动态光散射和ζ电位测量跟踪纳米颗粒的行为，观察到在该过程中纳米颗粒的尺寸没有显著变化，并且纳米颗粒的稳定性增加。用E.结果表明，Ag-PEG-ATCUN纳米复合物的活性高于纳米颗粒和ATCUN肽。
Strategies to design novel antibacterial materials may rely on the combination of materials to achieve synergistic effects. The coupling of antibacterial peptides to nanoparticles, however, needs to be directed conveniently to avoid structural changes within the peptide and/or degradation of the nanoparticle. Here, we present the results of the attachment of a synthetic peptide (VIHGW-alkyne-G-NH2) containing the amino terminal copper and nickel (ATCUN) motif to silver nanoparticles. In order to direct the peptide-nanoparticle coupling, the peptide was functionalized with an alkyne, whereas the nanoparticles were functionalized with azide groups using thiol-polyethylene glycol-azide (HS-PEG-N-3) chains, so that the acetylide and the azide can undergo a click reaction. The reaction was conducted at room temperature and the steps in the construction of the nanoparticle-PEG-ATCUN array were followed by a combination of UV-Vis absorption spectroscopy, X-ray photoelectron spectroscopy (XPS), and infrared spectroscopy. Evidence of the attachment of the PEG molecules through the thiol termination indicates that the nanoparticle is functionalized with azide groups, although only partially. The click reaction with the synthetic peptide is evidenced by the loss of the N-3-vibrational signal with infrared spectroscopy. Throughout the steps of the synthesis, the behavior of the nanoparticles was followed by UV-Vis spectroscopy, dynamic light scattering, and zeta potential measurements, observing that during the process there are no significant changes in the size of the nanoparticle and that the stability of the nanoparticles increases. Antibacterial tests, conducted using E. coli, showed that the activity of the Ag-PEG-ATCUN nanocomposites is higher than that of nanoparticles and ATCUN peptides separately.Graphical abstract