Eccentric exercise induces nitric oxide synthase expression through nuclear factor-κB modulation in rat skeletal muscle
Eccentric exercise induces nitric oxide synthase expression through nuclear factor-κB modulation in rat skeletal muscle
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DOI：
10.1152/japplphysiol.00816.2009
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发表时间：
2010-03-01
影响因子：
3.3
通讯作者：
Gonzalez-Gallego, Javier
中科院分区：
文献类型：
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作者：
Lima-Cabello, Elena;Cuevas, Maria J.;Gonzalez-Gallego, Javier
Lima-Cabello E，Cuevas MJ，Garatachea N，Baldini M，Almar M，Gonzalez-Gallego J.偏心运动通过大鼠骨骼肌核因子-κ B调节诱导一氧化氮合酶表达。J Appl Physiol 108：575-583，2010.首次发表于2009年12月31日; doi：10.1152/japplphysiol.00816.2009。研究离心运动对大鼠股外侧深肌一氧化氮合酶（NOS）不同亚型表达的影响。将24只大鼠分为4个实验组：安静对照组、间歇性下坡90 min后急性运动组、急性运动前24 h和1 h给予吡咯烷二硫代氨基甲酸酯（100 mg/kg ip）的急性运动组、急性运动前8 wk进行次极量离心训练的急性运动组。剧烈运动的大鼠显示蛋白质酪氨酸硝化、NF-κ B结合和磷酸化I β B α含量水平增加。神经元型NOS、诱导型NOS和内皮型NOS的mRNA水平和蛋白含量均显著增加。NF-κ B B与NOS亚型启动子的结合，通过染色质免疫沉淀法测定，在休息的大鼠中检测不到，而在急性运动的动物中很明显。所有这些影响被部分取消吡咯烷二硫代氨基甲酸酯治疗和训练。总之，我们的研究结果提供了一个直接的联系NF-κ B B信号级联反应和NOS表达在骨骼肌离心运动后，并建议调制的三个NOS亚型的表达，这种转录因子。
Lima-Cabello E, Cuevas MJ, Garatachea N, Baldini M, Almar M, Gonzalez-Gallego J. Eccentric exercise induces nitric oxide synthase expression through nuclear factor-kappa B modulation in rat skeletal muscle. J Appl Physiol 108: 575-583, 2010. First published December 31, 2009; doi:10.1152/japplphysiol.00816.2009.-This study aimed to investigate the effect of eccentric exercise on the expression of the different nitric oxide synthase (NOS) isoforms in rat deep vastus lateralis muscle. Twenty-four rats were allocated to four experimental groups: rested control group, acutely exercised group after an intermittent downhill protocol for 90 min, acutely exercised group treated with pyrrolidine dithiocarbamate (100 mg/kg ip) for 24 and 1 h before the acute exercise bout, and acutely exercised group with a previous submaximal eccentric training of 8 wk. Acutely exercised rats showed increased levels of protein tyrosine nitration, NF-kappa B binding, and phospho-I beta B alpha content. A significant increase was observed in mRNA level and protein content of neuronal NOS, inducible NOS, and endothelial NOS. The binding of NF-kappa B to the NOS isoform promoters, measured by a chromatin immunoprecipitation assay, was undetectable in rested rats, whereas it was evident in acutely exercised animals. All of these effects were partially abolished by pyrrolidine dithiocarbamate treatment and by training. In summary, our findings provide a direct link between the NF-kappa B signaling cascade and NOS expression in skeletal muscle following eccentric exercise and suggest a modulation of the expression of the three NOS isoforms by this transcription factor.