SNHG3 correlates with malignant status and poor prognosis in hepatocellular carcinoma
SNHG3 correlates with malignant status and poor prognosis in hepatocellular carcinoma
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DOI：
10.1007/s13277-015-4052-4
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发表时间：
2016-02-01
期刊：
影响因子：
--
通讯作者：
Liu, Li
中科院分区：
文献类型：
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作者：
Zhang, Ting;Cao, Chuanhui;Liu, Li
人们发现长非编码 RNA (lncRNA) 在人类疾病中失调，尤其是在癌症中。小核仁RNA宿主基因3（SNHG3）是一种lncRNA，其在肝细胞癌（HCC）中的潜在功能和机制仍然很大程度上未知。在本研究中，我们旨在确定 SNHG3 在 HCC 中的表达及其临床意义。我们的结果显示，通过定量实时聚合酶链反应（qRT-PCR；P < 0.001）测定，与 51 名 HCC 患者的配对非癌组织相比，HCC 组织中 SNHG3 的表达水平显着上调，这与癌症基因组图谱（TCGA）和 Oncomine 数据库中两个独立 HCC 队列的结果一致（P < 0.0001 和 P = 0.0325， 分别）。这些结果通过原位杂交测定 (ISH) 在 144 对石蜡包埋 HCC 标本中得到进一步证实。此外，SNHG3表达与肿瘤大小（P = 0.003）、门静脉癌栓（PVTT；P = 0.014）和复发（P = 0.038）显着相关。 SNHG3 的高表达水平与总生存期（OS；P < 0.0001）、无复发生存期（RFS；P = 0.006）和无病生存期（DFS；P < 0.0001）显着相关。更重要的是，多变量分析表明SNHG3表达是HCC患者的独立预后因素（P < 0.001）。总之，SNHG3 表达增加与 HCC 患者的恶性状态和不良预后相关。
Long noncoding RNAs (lncRNAs) have been found dysregulated in human disease, especially in cancer. Small nucleolar RNA host gene 3 (SNHG3) is an lncRNA whose potential function and mechanism in hepatocellular carcinoma (HCC) remain largely unknown. In the present study, we aimed to determine SNHG3 expression and its clinical significance in HCC. Our results showed that the expression level of SNHG3 was significantly upregulated in HCC tissues compared with paired noncancerous tissues from 51 HCC patients, as determined by quantitative real-time polymerase chain reaction (qRT-PCR; P < 0.001), which was consistent with the results of two independent HCC cohorts from The Cancer Genome Atlas (TCGA) and Oncomine databases (P < 0.0001 and P = 0.0325, respectively). These results were further confirmed in 144 paired paraffin-embedded HCC specimens by in situ hybridization assay (ISH). Furthermore, SNHG3 expression was significantly correlated with tumor size (P = 0.003), portal vein tumor thrombus (PVTT; P = 0.014), and relapse (P = 0.038). The high expression level of SNHG3 was markedly correlated with overall survival (OS; P < 0.0001), recurrence-free survival (RFS; P = 0.006), and disease-free survival (DFS; P < 0.0001). More importantly, multivariate analysis indicated that SNHG3 expression was an independent prognostic factor for HCC patients (P < 0.001). In conclusion, increased SNHG3 expression is associated with malignant status and poor prognosis in HCC patients.