Experimentally induced recruitment of plasmacytoid (CD123high) dendritic cells in human nasal allergy
Experimentally induced recruitment of plasmacytoid (CD123high) dendritic cells in human nasal allergy
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DOI：
10.4049/jimmunol.165.7.4062
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发表时间：
2000-10-01
影响因子：
4.4
通讯作者：
Brandtzaeg, P
中科院分区：
文献类型：
--
作者：
Jahnsen, FL;Lund-Johansen, F;Brandtzaeg, P
最近的证据表明，以前被称为浆细胞样单核细胞的神秘细胞类型可以作为树突状细胞发挥作用，对先天性免疫和获得性免疫都有很大贡献。这种细胞类型以前只在骨髓、血液和有组织的淋巴组织中被描述，但不在直接暴露于Ag的效应部位，如粘膜，在体外成熟的血浆细胞样树突状细胞(P-DC)可以诱导T细胞产生促进过敏的Th2细胞因子；因此，在实验诱导的变应性鼻炎期间，它们可能出现在鼻黏膜中。对无症状的鼻部过敏患者，每日局部注射相关过敏原，连续7天。在激发前和激发过程中取活检标本和血液标本，通过CD123(IL3Rα链)和CD45RA的高表达来鉴定P-DC。结果表明，在正常鼻粘膜中，P-DC数量很少且数量可变，但在变态反应过程中显著增加。这种积聚与粘膜血管内皮细胞上L-选择素配体外周淋巴结位素的表达一致。考虑到循环P-DC前体细胞上L-选择素的高水平，以及先前的报道表明这些细胞通过高内皮微静脉进入有组织的淋巴组织(这些微静脉结构性地表达外周淋巴结地址)，后一种观察尤其有趣。综上所述，我们的发现表明，P-DC参与了呼吸道过敏的触发，它们通过黏附分子定向到变态反应性损害，这些黏附分子通常介导有组织的淋巴组织中的白细胞外渗。
Recent evidence suggests that the previously enigmatic cell type designated plasmacytoid monocytes can function as dendritic cells and contribute substantially to both innate and adaptive immunity. This cell type has previously been described only in bone marrow, blood, and organized lymphoid tissue, but not at effector sites with direct Ag exposure such as the mucosae, Plasmacytoid dendritic cells (P-DCs) matured in vitro can induce T cells to produce allergy-promoting Th2 cytokines; therefore, their possible occurrence in nasal mucosa during experimentally elicited allergic rhinitis was examined. Patients with silent nasal allergy were challenged topically with relevant allergen daily for 7 days. Biopsy specimens as well as blood samples were obtained before and during such provocation, and P-DCs were identified by their high expression of CD123 (IL3R alpha-chain), together with CD45RA, Our results showed that P-DCs were present in low and variable numbers in normal nasal mucosa but increased dramatically during the allergic reaction. This accumulation concurred with the expression of the L-selectin ligand peripheral lymph node addressin on the mucosal vascular endothelium. The latter observation was particularly interesting in view of the high levels of L-selectin on circulating P-DC precursors and of previous reports suggesting that these cells ran enter organized lymphoid tissue via high endothelial venules (which express peripheral lymph node addressin constitutively). Together, our findings suggested that P-DCs are involved in the triggering of airway allergy and that they are directed to allergic lesions by adhesion molecules that normally mediate leukocyte extravasation in organized lymphoid tissue.