High frequency of hypermutable Pseudomonas aeruginosa in cystic fibrosis lung infection
High frequency of hypermutable Pseudomonas aeruginosa in cystic fibrosis lung infection
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DOI：
10.1126/science.288.5469.1251
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发表时间：
2000-05-19
期刊：
影响因子：
56.9
通讯作者：
Blázquez, J
中科院分区：
文献类型：
--
作者：
Oliver, A;Cantón, R;Blázquez, J
囊性纤维化（CF）患者的肺部被一种或几种铜绿假单胞菌谱系慢性感染多年。这些细菌群体适应CF患者的高度区室化和解剖学恶化的肺环境，以及免疫防御和抗生素治疗的挑战。这些选择性条件正是最近的理论研究预测的增加变异率的机制的演变。对来自30例CF患者的128株铜绿假单胞菌分离株的自发突变率进行测定，结果显示36%的患者被高变（增变）菌株定殖，该菌株在大多数患者中持续多年。75例非CF患者急性铜绿假单胞菌感染中未发现突变株。这项研究还揭示了体内高突变率与抗生素耐药性演变之间的联系。
The lungs of cystic fibrosis (CF) patients are chronically infected for years by one or a few Lineages of Pseudomonas aeruginosa. These bacterial populations adapt to the highly compartmentalized and anatomically deteriorating Lung environment of CF patients, as well as to the challenges of the immune defenses and antibiotic therapy, These selective conditions are precisely those that recent theoretical studies predict for the evolution of mechanisms that augment the rate of variation. Determination of spontaneous mutation rates in 128 P. aeruginosa isolates from 30 CF patients revealed that 36% of the patients were colonized by a hypermutable (mutator) strain that persisted for years in most patients. Mutator strains were not found in 75 non-CF patients acutely infected with P. aeruginosa. This investigation also reveals a link between high mutation rates in vivo and the evolution of antibiotic resistance.