Protein nanoarrays generated by dip-pen nanolithography
Protein nanoarrays generated by dip-pen nanolithography
复制标题
DOI：
10.1126/science.1067172
复制
发表时间：
2002-03-01
期刊：
影响因子：
56.9
通讯作者：
Mrksich, M
中科院分区：
文献类型：
--
作者：
Lee, KB;Park, SJ;Mrksich, M
浸笔纳米光刻用于构建具有 100 至 350 纳米特征的蛋白质阵列。即使在复杂的蛋白质混合物中，这些纳米阵列也几乎没有表现出可检测到的蛋白质与其钝化部分的非特异性结合，因此提供了研究各种表面介导的生物识别过程的机会。例如，涉及复杂溶液中蛋白质特征和抗原的反应可以通过原子力显微镜轻松筛选。作为进一步的概念验证，这些阵列用于研究亚微米尺度的细胞粘附。
Dip-pen nanolithography was used to construct arrays of proteins with 100- to 350-nanometer features. These nanoarrays exhibit almost no detectable nonspecific binding of proteins to their passivated portions even in complex mixtures of proteins, and therefore provide the opportunity to study a variety of surface-mediated biological recognition processes. For example, reactions involving the protein features and antigens in complex solutions can be screened easily by atomic force microscopy. As further proof-of-concept, these arrays were used to study cellular adhesion at the submicrometer scale.