The formation and stability of the hypusine containing protein in Chinese hamster ovary cells.
The formation and stability of the hypusine containing protein in Chinese hamster ovary cells.
复制标题
中国仓鼠卵巢细胞中含马尿苷蛋白的形成和稳定性。
DOI：
10.1016/0006-291x(87)91584-1
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Gallop,PM
中科院分区：
文献类型：
--
作者：
Torrelio,BM;Paz,MA;Gallop,PM
在对数生长的中国仓鼠卵巢细胞中，被鉴定为真核生物起始翻译因子 4D 的含马尿嘧啶的蛋白质被 [14C] 亚精胺标记。通过聚丙烯酰胺凝胶电泳分离的细胞蛋白的放射自显影显示 18000 Mr 的单个蛋白中存在标记。时程分析表明该蛋白在合成后长达 72 小时内保持未降解。酸水解后分离出的氨基酸hypusine 中存在的放射性在同一时间段内保持恒定。这些结果表明含有马尿苷的蛋白质具有长的半衰期。
Hypusine-containing protein identified as eukaryote initiationtranslation factor 4D was labeled with [14C]spermidine in logarithmically growing Chinese hamster ovary cells. Radioautography of the cellular proteins separated by polyacrylamide gel electrophoresis showed the label in a single protein of 18000 Mr. Time course analysis showed that this protein remained undegraded for up to 72 hours after its synthesis. Radioactivity present in the amino acid hypusine, isolated after acid hydrolysis, remained constant during the same period of time. These results indicate that the hypusine-containing protein has a long half-life.