The Role of ABA in the Maturation and Postharvest Life of a Nonclimacteric Sweet Cherry Fruit
The Role of ABA in the Maturation and Postharvest Life of a Nonclimacteric Sweet Cherry Fruit
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DOI：
10.1007/s00344-013-9388-7
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发表时间：
2014-06-01
影响因子：
4.8
通讯作者：
Leng, Ping
中科院分区：
文献类型：
--
作者：
Luo, Hao;Dai, ShengJie;Leng, Ping
本研究旨在探讨脱落酸(ABA)对非跃变型甜樱桃采前和采后成熟的影响。结果表明，ABA含量在稻草色阶段迅速增加，并在商品采收期前4d达到最高水平。ABA水平的增加与参与ABA合成的基因[9-cis-EPOXYCAROTENOID双加氧酶(PacNCED1)]的表达增加是平行的。早在ABA升高之前，参与ABA分解代谢的基因(PacCYP707A2)的表达就下调了。在稻草色期间，外源ABA促进了ABA的积累，促进了花青素的生物合成，提高了果实的成熟度指数(SSC/TA)，从而促进了果实的成熟。ABA处理促进了PacNCED1和参与花青素生物合成的基因(PacCHS、PacF3H、Pacchi、PacDFR和PacUFGT)的表达。NiCl2处理抑制了果实乙烯释放和编码1-氨基环丙烷-1-羧酸氧化酶的PacACO1的表达，其对果实成熟的影响与ABA相反。乙烯利和去甲二氢愈创木酸处理对果实成熟无影响。对于采后果实，花梗是失水的主要途径，脱水诱导了PacNCED1的转录和ABA的积累。随着乙烯的释放，果实采后衰老的开始被触发。综上所述，内源ABA通过调节花青素积累等与果实成熟相关的代谢途径来触发果实的成熟。ABA还通过促进樱桃果实乙烯释放而诱导果实采后衰老。
The purpose of this study was to investigate the role of abscisic acid (ABA) in the pre-and postharvest maturation of the nonclimacteric sweet cherry. It was found that ABA content increased rapidly at the straw-colored stage and reached its highest level 4 days before commercial harvest time. The increase in ABA level was paralleled by an increase in the expression of a gene involved in ABA synthesis [9-cis-epoxycarotenoid dioxygenase (PacNCED1)]. Well before the ABA increase, the expression of a gene involved in ABA catabolism (PacCYP707A2) was downregulated. During the straw-colored stage, the application of exogenous ABA induced ABA accumulation, anthocyanin biosynthesis, and an increase in the maturity index (SSC/TA), thereby promoting fruit ripening. ABA treatment stimulated the expression of PacNCED1 and of genes involved in anthocyanin biosynthesis (PacCHS, PacF3H, PacCHI, PacDFR, and PacUFGT). The application of NiCl2 inhibited the release of ethylene and the expression of PacACO1 encoded 1-aminocyclopropane- 1-carboxylic acid oxidase, of which the effect on fruit ripening was opposite that of ABA. Ethephon and nordihydroguaiaretic acid treatments, however, had no effect on fruit ripening. For postharvest fruit, the pedicel was a major route for water loss, and dehydration induced the transcription of PacNCED1 and the accumulation of ABA. Followed by ethylene release, the start of postharvest fruit senescence is triggered. In conclusion, endogenous ABA triggered the ripening of fruit via the modulation of ripening-related metabolism pathways such as anthocyanin accumulation. ABA also induced postharvest fruit senescence through stimulation of ethylene release in cherry fruit.