THE USE OF THE FIREFLY BIOLUMINESCENT REACTION FOR THE RAPID DETECTION AND COUNTING OF MYCOBACTERIUM BCG
THE USE OF THE FIREFLY BIOLUMINESCENT REACTION FOR THE RAPID DETECTION AND COUNTING OF MYCOBACTERIUM BCG
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DOI：
10.1016/0092-1157(87)90012-6
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发表时间：
1987-01-01
期刊：
影响因子：
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通讯作者：
SITKIEWICZ, D
中科院分区：
文献类型：
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作者：
JANASZEK, W;ALEKSANDROWICZ, J;SITKIEWICZ, D
本文介绍了一种以萤火虫荧光素酶法测定细菌ATP为基础的卡介苗快速计数试验。研究了三种不同的从分枝杆菌细胞中提取ATP的方法。正丁醇萃取法是最佳的提取方法。ATP的提取量与菌落形成单位的数量相关性良好。结果发现，在不同的培养时间后，BCG的每个菌落形成颗粒的ATP含量仅略有变化。
A rapid test for the counting of Mycobacterium BCG, based on firefly luciferase assay of bacterial ATP has been evaluated. Three different methods for the extraction of ATP form mycobacterial cells were examined. Extraction with n-butanol proved to be the best method. The amount of ATP extracted correlated well with the number of colony forming units. It was found that the ATP content per colony forming particle of BCG varied only slightly after various periods of cultivation.