Effect of the degree of phosphate substitution in aluminum hydroxide adjuvant on the adsorption of phosphorylated proteins
Effect of the degree of phosphate substitution in aluminum hydroxide adjuvant on the adsorption of phosphorylated proteins
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DOI：
10.1081/pdt-120017526
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发表时间：
2003-01-01
影响因子：
3.4
通讯作者：
Hem, SL
中科院分区：
文献类型：
--
作者：
Iyer, S;HogenEsch, H;Hem, SL
用六种浓度的磷酸二氢钾对氢氧化铝佐剂进行预处理，制得一系列表面羟基被不同程度磷酸盐取代的佐剂。研究了磷酸处理的氢氧化铝佐剂对三种磷酸化蛋白（α酪蛋白、去磷酸化α酪蛋白和卵清蛋白）的吸附。即使在静电斥力存在的情况下，磷酸化的蛋白质也被磷酸与羟基的配体交换所吸附。然而，吸附的程度（吸附容量）和强度（吸附系数）与氢氧化铝佐剂的磷酸盐取代程度成反比。应尽量减少含有氢氧化铝佐剂和磷酸化抗原的疫苗在配方或生产过程中与磷酸盐离子的接触，以最大限度地吸附抗原。
Aluminum hydroxide adjuvant was pretreated with six concentrations of potassium dihydrogen phosphate to produce a series of adjuvants with various degrees of phosphate substitution for surface hydroxyl. The adsorption of three phosphorylated proteins (alpha casein, dephosphorylated alpha casein, and ovalbumin) by the phosphate-treated aluminum hydroxide adjuvants was studied. The phosphorylated proteins were adsorbed by ligand exchange of phosphate for hydroxyl even when an electrostatic repulsive force was present. However, the extent (adsorptive capacity) and strength (adsorptive coefficient) of adsorption was inversely related to the degree of phosphate substitution of the aluminum hydroxide adjuvant. Exposure of vaccines containing aluminum hydroxide adjuvant and phosphorylated antigens to phosphate ion in the formulation or during manufacture should be minimized to produce maximum adsorption of the antigen.