(18)F-Trifluoromethylated D-Cysteine as a Promising New PET Tracer for Glioma Imaging: Comparative Analysis With MRI and Histopathology in Orthotopic C6 Models.
(18)F-Trifluoromethylated D-Cysteine as a Promising New PET Tracer for Glioma Imaging: Comparative Analysis With MRI and Histopathology in Orthotopic C6 Models.
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F-18-三氟甲基化 D-半胱氨酸作为一种有前途的新型 PET 示踪剂用于胶质瘤成像：在原位 C6 模型中与 MRI 和组织病理学进行比较分析
DOI：
10.3389/fonc.2021.645162
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发表时间：
2021
影响因子：
4.7
通讯作者：
Tang G
中科院分区：
文献类型：
--
作者：
Ma H;Zhao J;Liu S;Xie D;Zhang Z;Nie D;Wen F;Yang Z;Tang G
本研究通过原位C6胶质瘤模型，对比磁共振成像（MRI）和组织病理学，旨在全面探索S - [18F]三氟甲基化D - 半胱氨酸（S - [18F]CF3 - D - CYS）在评估胶质瘤方面的潜在应用。将C6胶质瘤细胞植入9只斯普拉格 - 道利（SD）大鼠体内。每周通过多参数MRI（包括动态对比增强MRI（DCE - MRI））、[18F]氟脱氧葡萄糖（[18F]FDG）、S - [18F]CF3 - D - CYS以及[18F]氟多巴（[18F]FDOPA）正电子发射断层扫描（PET）成像监测肿瘤生长。对同一只大鼠使用两种或三种[18F]标记的放射性示踪剂进行重复扫描研究。在T2加权像（T2WI）上手动勾勒出初始感兴趣区域，并在相同层面的PET图像上设置该区域，计算肿瘤与正常脑组织摄取比值（TNRs），以半定量评估示踪剂在肿瘤中的积聚情况。计算PET成像和组织病理学中的肿瘤体积。进一步进行苏木精 - 伊红（HE）染色和Ki67免疫组化染色。分析S - [18F]CF3 - D - CYS摄取量与Ki67之间的相关性。动态S - [18F]CF3 - D - CYS PET成像显示肿瘤摄取量迅速达到峰值，在注射后10 - 30分钟内保持平台期，然后缓慢下降。与[18F]FDG和[18F]FDOPA PET成像相比，S - [18F]CF3 - D - CYS PET显示出最高的TNRs（P < 0.05）。S - [18F]CF3 - D - CYS PET成像或HE标本所测肿瘤体积无显著差异。此外，我们的结果表明，S - [18F]CF3 - D - CYS的摄取量与肿瘤Ki67呈显著正相关，S - [18F]CF3 - D - CYS摄取量低与肿瘤出血情况相符。S - [18F]CF3 - D - CYS摄取量与DCE - MRI得出的Ktrans值之间无显著相关性。与MRI和组织病理学相比，S - [18F]CF3 - D - CYS PET在胶质瘤的诊断和评估方面表现良好。S - [18F]CF3 - D - CYS PET可能成为胶质瘤临床治疗中的一种有价值的工具。
Comparing MRI and histopathology, this study aims to comprehensively explore the potential application of 18F-trifluoromethylated D-cysteine (S-[18F]CF3-D-CYS) in evaluating glioma by using orthotopic C6 glioma models. Sprague–Dawley (SD) rats (n = 9) were implanted with C6 glioma cells. Tumor growth was monitored every week by multiparameter MRI [including dynamic contrast-enhanced MRI (DCE-MRI)], [18F]FDG, S-[18F]CF3-D-CYS, and [18F]FDOPA PET imaging. Repeated scans of the same rat with the two or three [18F]-labeled radiotracers were investigated. Initial regions of interest were manually delineated on T2WI and set on the same level of PET images, and tumor-to-normal brain uptake ratios (TNRs) were calculated to semiquantitatively assess the tracer accumulation in the tumor. The tumor volume in PET and histopathology was calculated. HE and Ki67 immunohistochemical staining were further performed. The correlations between the uptake of S-[18F]CF3-D-CYS and Ki67 were analyzed. Dynamic S-[18F]CF3-D-CYS PET imaging showed tumor uptake rapidly reached a peak, maintained plateau during 10–30 min after injection, then decreased slowly. Compared with [18F]FDG and [18F]FDOPA PET imaging, S-[18F]CF3-D-CYS PET demonstrated the highest TNRs (P < 0.05). There were no significant differences in the tumor volume measured on S-[18F]CF3-D-CYS PET or HE specimen. Furthermore, our results showed that the uptake of S-[18F]CF3-D-CYS was significantly positively correlated with tumor Ki67, and the poor accumulated S-[18F]CF3-D-CYS was consistent with tumor hemorrhage. There was no significant correlation between the S-[18F]CF3-D-CYS uptakes and the Ktrans values derived from DCE-MRI. In comparison with MRI and histopathology, S-[18F]CF3-D-CYS PET performs well in the diagnosis and evaluation of glioma. S-[18F]CF3-D-CYS PET may serve as a valuable tool in the clinical management of gliomas.
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DOI：
10.1007/s00259-018-4107-z
发表时间：
2019-03
影响因子：
9.1
作者：
Roodakker KR;Alhuseinalkhudhur A;Al-Jaff M;Georganaki M;Zetterling M;Berntsson SG;Danfors T;Strand R;Edqvist PH;Dimberg A;Larsson EM;Smits A
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影响因子：
2.6
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通讯作者：
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DOI：
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发表时间：
2020-01-01
影响因子：
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DOI：
10.1007/s00259-004-1598-6
发表时间：
2005-01-01
影响因子：
9.1
作者：
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通讯作者：
Lee, MC
影响因子：
4.9
作者：
Chiotellis, Aristeidis;Muller, Adrienne;Ametamey, Simon M.
通讯作者：
Ametamey, Simon M.