Species-specific diagnostics using a β-1,4-endoglucanase gene for Pratylenchus spp. occurring in the Pacific Northwest of North America
Species-specific diagnostics using a β-1,4-endoglucanase gene for Pratylenchus spp. occurring in the Pacific Northwest of North America
复制标题
使用 β-1,4-内切葡聚糖酶基因对北美太平洋西北地区的短体线虫属进行物种特异性诊断。
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
I. Zasada
中科院分区：
文献类型：
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作者：
A. Peetz;I. Zasada
设计并优化了一种PCR检测方法，以区分北美西北太平洋地区土壤和根样品中常见的四种短体线虫。对GenBank中已登录的短体线虫属（Pratylenchus）编码β-1，4-内切葡聚糖酶（β-1，4-endoglucanase，β-1，4-endoglucanase）基因的材料设计特异性引物。优化的β-1，4-内切葡聚糖酶基因引物组分别从钝齿拟青霉、疏忽拟青霉、反式拟青霉和索氏拟青霉中产生380、293、528和364 bp的扩增子。成功地测试了引物组的功能性和特异性内的四个物种，以及对其他物种不常见于太平洋西北部。这种方法允许识别幼鱼的物种，从而排除了准确诊断样本中存在雌性的必要性。最终，这种诊断PCR检测可以被用来作为一个有效的工具，这些短体线虫物种的快速诊断，从土壤和根样品中回收，在任何实验室配备PCR。
A PCR assay was designed and optimised to differentiate four Pratylenchus species commonly encountered in soil and root samples from the Pacific Northwest of North America. Species-specific primers were designed to accessions from Pratylenchus species deposited in GenBank which encoded a β -1,4-endoglucanase gene. The optimised β -1,4-endoglucanase gene primer sets produced amplicons that were 380, 293, 528 and 364 bp from P. crenatus , P. neglectus , P. penetrans and P. thornei , respectively. Primer sets were tested successfully for functionality and specificity within each of the four species as well as against other species not commonly found in the Pacific Northwest. This method allowed for the identification of juveniles to species, thereby precluding the necessity of the presence of females in a sample for accurate diagnostics. Ultimately, this diagnostic PCR assay could be used as an efficient tool for rapid diagnostics of these Pratylenchus species recovered from soil and root samples in any laboratory equipped for PCR.
影响因子：
1.2
作者：
Kanzaki, Natsumi;Giblin-Davis, Robin M.;Center, Barbara J.
通讯作者：
Center, Barbara J.