RAT SCHWANN-CELLS CAN BE INDUCED TO EXPRESS MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-II MOLECULES INVIVO
RAT SCHWANN-CELLS CAN BE INDUCED TO EXPRESS MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-II MOLECULES INVIVO
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DOI：
10.1007/bf01191706
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发表时间：
1992-05-01
期刊：
影响因子：
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通讯作者：
JESSEN, KR
中科院分区：
文献类型：
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作者：
BERGSTEINSDOTTIR, K;KINGSTON, A;JESSEN, KR
先前的研究表明，培养的大鼠雪旺细胞可以被诱导表达MHC II类分子，提出了在某些条件下，活神经中的雪旺细胞可能表达MHC II类分子并参与T淋巴细胞活化的可能性。在本工作中，髓鞘和非髓鞘形成的雪旺细胞在体内表达MHC II类分子的能力进行了研究。在注射干扰素-γ后三天或注射肿瘤坏死因子、麻风分枝杆菌抗原或整个麻风分枝杆菌抗原后六天，对神经进行检查，发现在注射部位附近的一些髓鞘形成的雪旺细胞上存在强烈的MHC II类免疫染色。极少数非髓鞘形成细胞表达MHC II类分子。注射的神经和毛细血管的内皮细胞表达高水平的MHC II类抗原的MHC II类阳性单核细胞。不注射因子而挤压坐骨神经也可诱导少数雪旺细胞表达MHC-II类分子，因此在体内和体外一样可诱导大鼠雪旺细胞表达MHC-II类分子。这增强了在活神经中雪旺细胞能够在T细胞介导的免疫应答的起始或增强中作为辅助细胞起作用的可能性。
Previous studies, showing that cultured rat Schwann cells could be induced to express MHC class II molecules, raised the possibility that Schwann cells in living nerves might, under some conditions, express MHC class II molecules and take part in activation of T lymphocytes. In the present work, the ability of myelin- and non-myelin-forming Schwann cells in vivo to express MHC class II molecules was investigated.Lymphokines or bacterial antigens were injected into the living sciatic nerve of adult rats. Examination of the nerves three days after injection of interferon-gamma or six days after injection of either tumour necrosis factor, antigens from mycobacterium leprae or whole mycobacteria leprae, revealed strong MHC class II immunostaining on some myelin-forming Schwann cells in the vicinity of the injection site. Very few non-myelin-forming cells expressed MHC class II molecules. MHC class II positive mononuclear cells were present in the injected nerves and endothelial cells of capillaries expressed high levels of MHC class II antigens. Crushing the sciatic nerve without injection of factors also induced MHC class II molecules on a few Schwann cells.Thus rat Schwann cells can be induced to express MHC class II molecules in vivo as in vitro. This strengthens the possibility that in living nerves Schwann cells are able to function as accessory cells in the initiation or augmentation of T cell-mediated immune responses.