Hydroxy fatty acid production by Pediococcus sp
Hydroxy fatty acid production by Pediococcus sp
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片球菌产生羟基脂肪酸
DOI：
10.1002/ejlt.201200414
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
J. Ogawa
中科院分区：
文献类型：
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作者：
Takeuchi;M.;S. Kishino;K. Tanabe;A. Hirata;S.B. Park;S. Shimizu;J. Ogawa
通过对约300株乳酸菌的筛选，初步确定乳酸菌为小球菌属。AKU 1080是一株能够将亚油酸(顺-9，顺-12-十八碳二烯酸)水合生成3种羟基脂肪酸的菌株，即10-羟基-顺-12-十八烯酸、13-羟基-顺-9-十八烯酸和10，13-二羟基十八烯酸。该菌株将Δ9和Δ12位的两个双键中的一个水合，分别生成10-羟基-顺-12-十八碳烯酸和13-羟基-顺-9-十八碳烯酸，然后将这两种单羟基脂肪酸进一步水合为10，13-二羟基十八烷酸。该菌株的生长细胞被应用于13-羟基-顺-9-十八烯酸的生产，该酸具有作为聚合物底物和功能食品的潜力，但其特异性和高效生产尚未建立。在最佳培养条件下，以12.3 mg/mL亚油酸和0.0 4 mg/mL10-羟基顺-12-十八烯酸和0.0 5 mg/mL10，13-二羟基十八烷酸为主要原料，可获得2.3 mg/mL13-羟基顺-9-十八烯酸。以该菌株的无细胞提取液为催化剂，实现了13-羟基-顺-9-十八碳烯酸的专一性生产。在最佳反应条件下，以不含羟基苯甲酸的亚油酸和10，13-二羟基十八烷酸为原料，可制得0.4 mg/mL的13-羟基-顺-9-十八烯酸。实际应用：羟基脂肪酸可作为化工原料、功能食品和医药原料。将微生物在不饱和脂肪酸中区域选择性地引入羟基应用于羟基脂肪酸的生产。特别是建立了13-羟基-顺-9-十八碳烯酸的专一性生产方法，为生产具有减肥活性的13-氧代脂肪酸奠定了基础。
Through the screening of about 300 strains of lactic acid bacteria,Pediococcussp. AKU 1080 was selected as a strain with the ability to hydrate linoleic acid (cis‐9,cis‐12‐octadecadienoic acid) to three hydroxy fatty acids, i.e., 10‐hydroxy‐cis‐12‐octadecenoic acid, 13‐hydroxy‐cis‐9‐octadecenoic acid, and 10,13‐dihydroxyoctadecanoic acid. The strain hydrated one of twocisdouble bonds at Δ9 and Δ12 positions to produce 10‐hydroxy‐cis‐12‐octadecenoic acid and 13‐hydroxy‐cis‐9‐octadecenoic acid, respectively, then further hydrated these two mono‐hydroxy fatty acids to 10,13‐dihydroxyoctadecanoic acid. The growing cells of this strain were applied to the production of 13‐hydroxy‐cis‐9‐octadecenoic acid, that is potential as polymer substrates and functional foods but its specific and efficient production was not established. Under the optimum conditions, 2.3 mg/mL of 13‐hydroxy‐cis‐9‐octadecenoic acid was produced from 12.3 mg/mL of linoleic acid with 0.04 mg/mL 10‐hydroxy‐cis‐12‐octadecenoic acid (HYA) and 0.05 mg/mL 10,13‐dihydroxyoctadecanoic acid in the cultivation medium. Specific production of 13‐hydroxy‐cis‐9‐octadecenoic acid was attained using cell‐free extracts of the strain as the catalyst. Under the optimum conditions, 0.4 mg/mL of 13‐hydroxy‐cis‐9‐octadecenoic acid was produced from 2.0 mg/mL of linoleic acid without HYA and 10,13‐dihydroxyoctadecanoic acid.Practical applications:Hydroxy fatty acids are useful as starting materials for industrial chemicals, functional foods, and pharmaceuticals. Regioselective introduction of hydroxyl group to unsaturated fatty acids by microorganisms was applied to hydroxy fatty acid production. Especially, specific production of 13‐hydroxy‐cis‐9‐octadecenoic acid, which is useful for the production of 13‐oxo‐fatty acids with anti‐obesity activity, was established in this work.