Effects of manipulating slowpoke calcium-dependent potassium channel expression on rhythmic locomotor activity in Drosophila larvae.
Effects of manipulating slowpoke calcium-dependent potassium channel expression on rhythmic locomotor activity in Drosophila larvae.
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作者：
McKiernan EC
有节奏的运动行为是由神经元网络产生的。肌肉收缩的顺序和时间取决于神经元之间的突触连接和神经元的内在属性。特别是，运动神经元离子电流可能对运动输出有显著贡献。大电导Ca ~（2+）依赖性K ~+（BK）电流在神经元动作电位复极、峰间期、重复放电和爆发放电、爆发终止和爆发间期等过程中起重要作用。编码BK通道的慢波（slowpoke）基因突变导致运动障碍。本研究以果蝇幼虫为模型系统，研究了操纵β通道表达对节律性运动活动的影响。双细胞内记录相邻的体壁肌肉自发爬行相关的活动在幼虫表达的一个突变或mRNA干扰结构。节律性活动的发生率和持续时间的突变体与野生型对照动物相似，而运动模式的时间被改变。相对于野生型，突变体的爆发持续时间、周期持续时间和静止间隔减少，占空比增加。在已鉴定的运动神经元中表达cDNARNAi表型模仿了在突变体中观察到的许多效应，包括静止间隔和周期持续时间的减少。总的来说，这些结果表明，改变整个幼虫，特别是运动神经元中的表达，会改变爬行活动的频率。这些结果表明，运动神经元的内在属性在塑造运动输出的时间的重要作用。
Rhythmic motor behaviors are generated by networks of neurons. The sequence and timing of muscle contractions depends on both synaptic connections between neurons and the neurons’ intrinsic properties. In particular, motor neuron ion currents may contribute significantly to motor output. Large conductance Ca2+-dependent K+ (BK) currents play a role in action potential repolarization, interspike interval, repetitive and burst firing, burst termination and interburst interval in neurons. Mutations in slowpoke (slo) genes encoding BK channels result in motor disturbances. This study examined the effects of manipulating slo channel expression on rhythmic motor activity using Drosophila larva as a model system. Dual intracellular recordings from adjacent body wall muscles were made during spontaneous crawling-related activity in larvae expressing a slo mutation or a slo RNA interference construct. The incidence and duration of rhythmic activity in slo mutants were similar to wild-type control animals, while the timing of the motor pattern was altered. slo mutants showed decreased burst durations, cycle durations, and quiescence intervals, and increased duty cycles, relative to wild-type. Expressing slo RNAi in identified motor neurons phenocopied many of the effects observed in the mutant, including decreases in quiescence interval and cycle duration. Overall, these results show that altering slo expression in the whole larva, and specifically in motor neurons, changes the frequency of crawling activity. These results suggest an important role for motor neuron intrinsic properties in shaping the timing of motor output.
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影响因子：
5.5
作者：
Denton, M. Enriquez;Wienecke, J.;Kirkwood, P. A.
通讯作者：
Kirkwood, P. A.
影响因子：
1.9
作者：
Feng, YF;Ueda, A;Wu, CF
通讯作者：
Wu, CF
影响因子：
30.8
作者：
Du, W;Bautista, JF;Wang, QK
通讯作者：
Wang, QK
影响因子：
4.7
作者：
Brenner, R;Yu, JY;Atkinson, NS
通讯作者：
Atkinson, NS
DOI：
10.1111/j.1749-6632.1998.tb09057.x
发表时间：
1998-01-01
期刊：
NEURONAL MECHANISMS FOR GENERATING LOCOMOTOR ACTIVITY
影响因子：
--
作者：
Atkinson, NS;Brenner, R;Wilbur, JL
通讯作者：
Wilbur, JL