Two methods of temperature control for single-molecule measurements
Two methods of temperature control for single-molecule measurements
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DOI：
10.1007/s00249-010-0667-y
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发表时间：
2011-05-01
影响因子：
2
通讯作者：
Berry, Richard M.
中科院分区：
文献类型：
--
作者：
Baker, Matthew A. B.;Inoue, Yuichi;Berry, Richard M.
现代单分子生物物理实验需要与样品紧密接触的高数值孔径油浸物镜。我们介绍了两种可在任何显微镜上实现的高数值孔径温度控制方法：使用环形珀耳帖装置进行物镜温度控制，以及使用与样品紧密热接触的流体流冷却芯片进行载物台温度控制。我们通过显示两个分子马达（细菌鞭毛马达和骨骼肌肌球蛋白）的速度随温度的变化来证明每个系统的功效。
Modern single-molecule biophysical experiments require high numerical aperture oil-immersion objectives in close contact with the sample. We introduce two methods of high numerical aperture temperature control which can be implemented on any microscope: objective temperature control using a ring-shaped Peltier device, and stage temperature control using a fluid flow cooling chip in close thermal contact with the sample. We demonstrate the efficacy of each system by showing the change in speed with temperature of two molecular motors, the bacterial flagellar motor and skeletal muscle myosin.