αβ-Tubulin and Microtubule-Binding Assays
αβ-Tubulin and Microtubule-Binding Assays
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DOI：
10.1007/978-1-61779-252-6_6
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Slep, Keviin C.
中科院分区：
文献类型：
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作者：
Campbell, Jaime N.;Slep, Keviin C.
动态不稳定性是微管细胞骨架的标志。为了调节微管在体内的动力学，各种各样的微管相关蛋白被动员以促进微管成核、生长、稳定、突变、解聚、拯救和切断。为了赋予这些不同的功能，细胞骨架调节剂对微管蛋白具有高度调节的亲和力，识别未聚合的α β-微管蛋白异源二聚体、动态微管晶格、稳定的微管晶格或其中的组合。这里提出的协议探针α β-微管蛋白和微管结合使用凝胶过滤和共沉淀，分别。
Dynamic instability is a hallmark of the microtubule cytoskeleton. In order to regulate microtubule dynamics in vivo, a varied host of microtubule-associated proteins are mobilized to promote microtubule nucleation, growth, stabilization, catastrophe, depolymerization, rescue, and severing. To confer these various functions, cytoskeletal regulators have highly tuned affinities for tubulin, recognizing the unpolymerized alpha beta-tubulin heterodimer, dynamic microtubule lattice, stabilized microtubule lattice, or a combination therein. The protocols presented here probe alpha beta-tubulin and microtubule binding using gel filtration and cosedimentation, respectively.