Instrument to detect near-infrared fluorescence in solid-phase immunoassay.
Instrument to detect near-infrared fluorescence in solid-phase immunoassay.
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DOI：
10.1021/ac00091a018
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发表时间：
1994-10
影响因子：
7.4
通讯作者：
R. J. Williams;N. Narayanan;G. A. Casay;M. Lipowska;L. Strekowski,;G. Patonay;J. M. Peralta;V. Tsang
中科院分区：
文献类型：
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作者：
R. J. Williams;N. Narayanan;G. A. Casay;M. Lipowska;L. Strekowski,;G. Patonay;J. M. Peralta;V. Tsang
介绍了一种用于测量皮摩尔级近红外激光染料的近红外荧光检测器的结构。该检测器设计用于免疫测定技术，该技术采用近红外多甲川花青染料标记的抗体。这些染料具有专属于近红外区域（650-1100 nm）的光谱特性。该仪器的特点，包括其硬件和数据采集软件组成。该检测器能够测量溶液和固相环境中的荧光。检测器的性能数据。
The construction of a near-infrared (near-IR) fluorescence detector for measuring picomolar levels of near-IR laser dyes is described. The detector is designed for use in an immunoassay technique that employs antibodies labeled with near-IR polymethine cyanine dyes. These dyes possess spectral properties that are exclusive to the near-IR region (650-1100 nm). The instrumentation is characterized, including its hardware and data acquisition software components. The detector is capable of measuring fluorescence in both solution and solid-phase environments. Data on the detector's performance is presented.