Effects of storage time and freezing temperature on clinical chemical parameters from canine serum and heparinized plasma
Effects of storage time and freezing temperature on clinical chemical parameters from canine serum and heparinized plasma
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DOI：
10.1111/j.1939-165x.1995.tb00954.x
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发表时间：
1995-01-01
影响因子：
1.2
通讯作者：
Morberg, H.
中科院分区：
文献类型：
--
作者：
Thoresen, S. I.;Tverdal, A.;Morberg, H.
为了评估冷冻血清和肝素化血浆中24种血液成分的变化，从10只临床正常的德国牧羊犬中抽取血液样品。研究了9种酶（ALP、ALT、淀粉酶、AST、CK、GGT、GLDH、LDH、脂肪酶）和15种代谢产物和矿物质（白蛋白、胆汁酸、胆红素、钙、胆固醇、肌酐、果糖胺、葡萄糖、镁、磷酸盐、钾、蛋白质、钠、甘油三酯、尿素）的储存特性。血清和肝素化血浆的平行样本在两种不同的储存温度（-20 ° C和-70 ° C）下储存90天和240天。24种分析物中的16种（ALP、ALT、淀粉酶、AST、CK、GGT、GLDH、LDH、胆汁酸、钙、胆固醇、肌酐、果糖胺、镁、磷酸盐、尿素）在储存期间显示出与储存时间、储存温度和样本类型相关的统计学显著性（p lt 0.05）变化。7种分析物（淀粉酶、GGT、GLDH、LDH、胆汁酸、果糖胺、镁）显示可能具有临床意义的变化，储存期间酶较基线的平均差异大于20%，代谢物和矿物质较基线的平均差异大于10%。
To assess changes in 24 blood constituents in frozen serum and heparinized plasma, blood samples were drawn from 10 clinically normal German Shepherd army dogs. The storage characteristics of nine enzymes (ALP, ALT, amylase, AST, CK, GGT, GLDH, LDH, lipase), and 15 metabolites and minerals (albumin, bile acids, bilirubin, calcium, cholesterol, creatinine, fructosamine, glucose, magnesium, phosphate, potassium, protein, sodium, triglycerides, urea) were studied. Parallel samples of serum and heparinized plasma were stored for 90 and 240 days at two different storage temperatures, -20 degree C and -70 degree C. Sixteen of the 24 analytes (ALP, ALT, amylase, AST, CK, GGT, GLDH, LDH, bile acids, calcium, cholesterol, creatinine, fructosamine, magnesium, phosphate, urea) showed statistically significant (p lt 0.05) changes during the storage period related to storage time, storage temperature, and sample type. Seven of the analytes (amylase, GGT, GLDH, LDH, bile acids, fructosamine, magnesium) showed changes of possible clinical importance with mean differences from baseline larger than 20% for the enzymes and 10% for the metabolites and minerals during the storage periods.