Identification of a novel leucine-rich repeat protein as a component of flagellar radial spoke in the Ascidian Ciona intestinalis.
Identification of a novel leucine-rich repeat protein as a component of flagellar radial spoke in the Ascidian Ciona intestinalis.
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DOI：
10.1091/mbc.02-06-0089
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发表时间：
2003-02
影响因子：
3.3
通讯作者：
P. Padma;Yuhkoh Satouh;Ken-ichi Wakabayashi;Akiko Hozumi;Y. Ushimaru;R. Kamiya;K. Inaba
中科院分区：
文献类型：
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作者：
P. Padma;Yuhkoh Satouh;Ken-ichi Wakabayashi;Akiko Hozumi;Y. Ushimaru;R. Kamiya;K. Inaba
轴突体是一种在许多真核生物中保守的高度组织化的微管结构。为了用蛋白质组学的方法研究轴突蛋白，我们利用抗血清对海鞘睾丸轴突蛋白文库进行免疫筛选，选择性地克隆了轴突蛋白。我们在这里报告了一个37 kda蛋白，cDNA在所有阳性克隆中出现的频率最高。该蛋白被命名为LRR37，属于SDS22+ leucine-rich repeat （LRR）家族。LRR37不同于在衣藻和海胆鞭毛中报道的LRR外臂动力蛋白轻链，是一种新的轴突LRR蛋白。免疫电镜显示，LRR37定位于径向辐条尖端，极有可能位于辐条头部。事实上，在KI提取的轴突体中，LRR37蛋白似乎与径向辐状蛋白3形成复合物。这些结果表明，LRR37是径向辐头的一个组成部分，并参与与其他径向辐组件或中央对投射蛋白的相互作用。
Axonemes are highly organized microtubule-based structures conserved in many eukaryotes. In an attempt to study axonemes by a proteomics approach, we selectively cloned cDNAs of axonemal proteins by immunoscreening the testis cDNA library from the ascidian Ciona intestinalis by using an antiserum against whole axonemes. We report here a 37-kDa protein of which cDNA occurred most frequently among total positive clones. This protein, named LRR37, belongs to the class of SDS22+ leucine-rich repeat (LRR) family. LRR37 is different from the LRR outer arm dynein light chain reported in Chlamydomonas and sea urchin flagella, and thus represents a novel axonemal LRR protein. Immunoelectron microscopy by using a polyclonal antibody against LRR37 showed that it is localized on the tip of the radial spoke, most likely on the spoke head. The LRR37 protein in fact seems to form a complex together with radial spoke protein 3 in a KI extract of the axonemes. These results suggest that LRR37 is a component of the radial spoke head and is involved in the interaction with other radial spoke components or proteins in the central pair projection.