Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles
Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles
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DOI：
10.1063/1.3554415
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发表时间：
2011-02-28
影响因子：
4
通讯作者：
Dholakia, Kishan
中科院分区：
文献类型：
--
作者：
Arita, Yoshihiko;Torres-Mapa, Maria Leilani;Dholakia, Kishan
我们演示了用纳秒激光脉冲激光诱导的光捕获纳米粒子的击穿。在显微镜样品中实现了可控的空化，对活细胞单层产生剪切应力。这有效地渗透了它们的质膜。我们表明，与以前报道的方法相比，这种技术是一种极好的质粒dna转染细胞的工具，既降低了能量需求，又减少了细胞裂解。同时多位点靶向纳米手术细胞也证明了使用空间光调制器并行技术。(C) 2011年美国物理研究所。(doi: 10.1063/1.3554415)
We demonstrate laser-induced breakdown of an optically trapped nanoparticle with a nanosecond laser pulse. Controllable cavitation within a microscope sample was achieved, generating shear stress to monolayers of live cells. This efficiently permeabilize their plasma membranes. We show that this technique is an excellent tool for plasmid-DNA transfection of cells with both reduced energy requirements and reduced cell lysis compared to previously reported approaches. Simultaneous multisite targeted nanosurgery of cells is also demonstrated using a spatial light modulator for parallelizing the technique. (C) 2011 American Institute of Physics. [doi:10.1063/1.3554415]