Glial cell line-derived neurotrophic factor alters the growth characteristics and genomic imprinting of mouse multipotent adult germline stem cells
Glial cell line-derived neurotrophic factor alters the growth characteristics and genomic imprinting of mouse multipotent adult germline stem cells
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DOI：
10.1016/j.yexcr.2009.11.021
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发表时间：
2010-03-10
影响因子：
3.7
通讯作者：
Lee, Hoon Taek
中科院分区：
文献类型：
--
作者：
Jung, Yoon Hee;Gupta, Mukesh Kumar;Lee, Hoon Taek
本研究通过在GDNF、白血病抑制因子（LIF）或两者的存在下培养已建立的小鼠多能成年种系干细胞（maGS）细胞系，评估了胶质细胞系来源的神经营养因子（GDNF）在体外培养中的重要性。我们发现，在没有LIF的情况下，GDNF减缓了maGS细胞的增殖，导致更小的集落，而细胞在不同细胞周期阶段的分布、多能性基因的表达和体外分化潜力没有任何变化。GDNF增加了W/W-v突变小鼠雄性种系基因的表达，并在未形成畸胎瘤的情况下重新填充了空精小管。GDNF还改变了Igf2、Peg1和H19基因的基因组印迹，但对Oct4、Nanog和Stra8基因的DNA甲基化没有影响。然而，GDNF的这些作用在LIF的存在下被掩盖。在LIF存在的情况下，GDNF也不会干扰maGS细胞的多能性。综上所述，我们的研究结果表明，在LIF不存在的情况下，GDNF改变了maGS细胞的生长特性，并部分赋予了它们一些种系干细胞（GS）样细胞的特性。(c) 2009爱思唯尔公司版权所有
This study evaluated the essentiality of glial cell line-derived neurotrophic factor (GDNF) for in vitro culture of established mouse multipotent adult germline stem (maGS) cell lines by culturing them in the presence of GDNF, leukemia inhibitory factor (LIF) or both. We show that, in the absence of LIF, GDNF slows the proliferation of maGS cells and result in smaller sized colonies Without any change in distribution of cells to different cell-cycle stages, expression Of pluripotency genes and in vitro differentiation potential Furthermore, in the absence of LIF, GDNF increased the expression of male germ-line genes and repopulated the empty seminiferous tubule of W/W-v mutant mouse Without the formation of teratoma GDNF also altered the genomic imprinting Of Igf2, Peg1, and H19 genes but had no effect on DNA methylation of Oct4, Nanog and Stra8 genes. However, these effects of GDNF were masked in the presence of LIF. GDNF also did not interfere with the multipotency of maGS cells if they are cultured in the presence of LIF In conclusion, our results Suggest that, in the absence of LIF, GDNF alters the growth characteristics of maGS cells and partially impart them some of the germline stem (GS) cell-like characteristics. (c) 2009 Elsevier Inc. All rights reserved