Effects of hepatitis B virus S protein on human sperm function
Effects of hepatitis B virus S protein on human sperm function
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乙型肝炎病毒S蛋白对人精子功能的影响
DOI：
10.1093/humrep/dep050
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发表时间：
2009-07-01
影响因子：
6.1
通讯作者：
Liu, Li-Min
中科院分区：
文献类型：
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作者：
Zhou, Xiao-Ling;Sun, Ping-Nan;Liu, Li-Min
B型肝炎病毒（HBV）已被确定存在于慢性HBV感染患者的精液和男性生殖细胞中，但HBV S蛋白（HBV包膜蛋白的主要成分）对人类生殖系统的影响尚无数据。本文通过精子活力测定、精子穿透试验、线粒体膜电位测定、共聚焦显微镜免疫定位和流式细胞术分析，研究了HBs对人精子功能的影响，结果表明，HBs以剂量和时间依赖的方式降低精子活力，并引起精子线粒体膜电位的丧失。抗-HBs单克隆抗体（MAb）复合物明显加重了这种损害。25、50、100 μ g/ml的HBs孵育4 h后，精子活动率a+B均显著低于对照组（P < 0.01）。受精率和受精指数分别为40%和0.57，显著低于对照组的90%和1.6（P < 0.01）。去唾液酸糖蛋白受体（ASGP-R）和HBs主要定位于顶体后区。ASGP-R单克隆抗体和ASGP-R的高亲和力配体去唾液酸胎球蛋白均能抑制HBsAg引起的精子活力和线粒体膜电位的降低，表明HBsAg对精子功能有不良影响，ASGP-R可能在精子摄取HBs中起一定作用，ASGP-R单克隆抗体和去唾液酸胎球蛋白可竞争性抑制精子摄取HBs，从而减轻HBs对精子的损伤。
Hepatitis B virus (HBV) has been determined to exist in semen and male germ cells from patients with chronic HBV infection, but no data are yet available on the impact of HBV S protein (HBs), the main component of HBV envelop protein, on the human reproductive system. The purpose of this article was to investigate the effect of HBs on human sperm function.Sperm motility analyses, sperm penetration assays, mitochondrial membrane potential assays, immunolocalizations with confocal microscopy and flow cytometry analyses were performed.HBs reduced sperm motility in a dose- and time-dependent manner and caused the loss of sperm mitochondrial membrane potential. HBs-HBs monoclonal antibody (MAb) complex apparently aggravated such impairments. After 4 h incubation with HBs at concentrations of 25, 50, 100 mu g/ml, the percentages of sperm motility a+b significantly decreased compared with the control (P < 0.01). The fertilization rate and the fertilizing index in HBs-treated group were 40% and 0.57, respectively, which were significantly lower than 90% and 1.6, respectively, in the control (P < 0.01). The asialoglycoprotein receptor (ASGP-R) and HBs were found to localize mainly on the postacrosomal region. Both ASGP-R MAb and asialofoetuin, a high-affinity ligand of ASGP-R, inhibited the HBs-caused loss of sperm motility and mitochondrial membrane potential.HBs had adverse effects on human sperm function, and ASGP-R may play a role in the uptake of HBs into sperm cells, as demonstrated by the competitive inhibition of ASGP-R MAb or asialofoetuin, resulting in diminished impairment caused by HBs.