Extracorporeal shock wave therapy accelerates endochondral ossification and fracture healing in a rat femur delayed-union model
Extracorporeal shock wave therapy accelerates endochondral ossification and fracture healing in a rat femur delayed-union model
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DOI：
10.1016/j.bbrc.2020.07.084
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发表时间：
2020-10-01
影响因子：
3.1
通讯作者：
Yoshida, Kiyoshi
中科院分区：
文献类型：
--
作者：
Kobayashi, Masato;Chijimatsu, Ryota;Yoshida, Kiyoshi
体外冲击波疗法（ESWT）已被证明可以加速骨愈合；然而，eswt诱导骨再生的机制尚未完全阐明。本研究旨在探讨ESWT对骨折愈合过程的影响。采用烧灼骨膜法建立大鼠股骨延迟愈合模型。术后2周对骨折部位进行ESWT治疗，并在第4周、第6周和第8周对骨折部位进行影像学和组织学评估。8周时，ESWT组骨愈合率和影像学评分均显著高于对照组。组织学评价显示骨折部位软骨内成骨增强。体外观察ESWT对ATDC5细胞的影响。ESWT促进软骨细胞分化，但不抑制ATDC5细胞的增殖。ESWT可能通过促进骨折部位软骨内成骨而显著诱导骨愈合。(c) 2020 Elsevier Inc.版权所有。
Extracorporeal shock wave therapy (ESWT) has been demonstrated to accelerate bone healing; however, the mechanism underlying ESWT-induced bone regeneration has not been fully elucidated. This study aimed to examine the effects of ESWT and the process of fracture healing. A rat model of femur delayed union was established by cauterizing the periosteum. ESWT treatment at the fracture site was performed 2 weeks after the operation and the site was radiographically and histologically evaluated at weeks 4, 6, and 8. The bone union rate and radiographic score of the ESWT group were significantly higher than those of the control group at 8 weeks. Histological evaluation revealed enhanced endochondral ossification at the fracture site. The effects of ESWT on ATDC5 cells were examined in vitro. ESWT promoted chondrogenic differentiation without inhibiting the proliferation of ATDC5 cells. ESWT may induce significant bone healing by promoting endochondral ossification at the fracture site. (c) 2020 Elsevier Inc. All rights reserved.