Expression of Bone Morphogenetic Protein Receptor (BMPR) during Perinatal Ovary Development and Primordial Follicle Formation in the Hamster: Possible Regulation by FSH
Expression of Bone Morphogenetic Protein Receptor (BMPR) during Perinatal Ovary Development and Primordial Follicle Formation in the Hamster: Possible Regulation by FSH
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DOI：
10.1210/en.2008-0900
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发表时间：
2009-04-01
期刊：
影响因子：
4.8
通讯作者：
Roy, Shyamal K.
中科院分区：
文献类型：
--
作者：
Wang, Cheng;Roy, Shyamal K.
为了了解骨形态发生蛋白在金黄地鼠原始卵泡形成中的作用，我们研究了骨形态发生蛋白受体(BMPR)在胚胎(E)13天至出生后15天(P)卵巢细胞中的时空表达，以及FSH在P8的原始卵泡形成过程中可能的调节作用。在整个卵巢发育过程中，BMPRIA和BMPRII的mRNA水平显著高于BMPR1B。BMPRIA和BMPRII的mRNA水平在第14天显著升高，在第5天至第6天下降。随着原始卵泡的形成，BMPRII信使核糖核酸水平在P7时再次升高，BMPRIA信使核糖核酸水平在P8时升高。相反，BMPRIB的mRNA水平在P7-9时增加了10倍以上，在P10时又增加了3倍。BMPR蛋白在胚胎第13天在体细胞和卵母细胞中含量较低，但在出生后发育过程中呈递增趋势。P8时BMPR在体细胞的表达明显增加。而BMPRII的表达在P10后下降，此后保持稳定，而BMPRIA蛋白的表达一直波动到P15，然后变得低而稳定。总体而言，BMPRIB的免疫反应性也在P10时下降，然后在P15期间保持在间质细胞中的低水平。FSH抗血清对E12的抑制作用明显减弱P8的受体mRNA和蛋白水平，而马绒毛膜促性腺激素替代对P1的抑制作用被逆转。此外，FSH在体外可上调P4卵巢的BMPR水平。在围产期卵巢发育过程中，BMPR在卵母细胞和体细胞中表达的这种独特模式表明，BMP可能在原始卵泡的形成中起着调节作用。此外，FSH可能通过调节其受体的表达来调节BMP的作用。(内分泌学150：1886-1896,2009)
To understand whether bone morphogenetic protein plays any role in the formation of primordial follicles in the hamster, we examined the temporal and spatial expression of bone morphogenetic protein receptor (BMPR) mRNA and protein in embryonic (E) 13 through postnatal day (P) 15 ovarian cells and a possible regulation by FSH during the formation of primordial follicles on P8. BMPRIA and BMPRII mRNA levels were significantly higher than that of BMPR1B throughout ovary development. BMPRIA and BMPRII mRNA levels increased significantly on E14 and declined by P5 through P6. Whereas BMPRII mRNA increased again by P7, BMPRIA mRNA levels increased through P8 concurrent with primordial follicle formation. In contrast, BMPRIB mRNA levels increased greater than 10-fold on P7-9, with a further 3-fold increase by P10. BMPR proteins were low in the somatic cells and oocytes on E13 but increased progressively during postnatal development. BMPR expression in somatic cells increased markedly on P8. Whereas BMPRII expression declined by P10 and remained steady thereafter, BMPRIA protein expression fluctuated until P15 when it became low and steady. Overall, BMPRIB immunoreactivity also declined by P10 and then remained low in the interstitial cells through P15. FSH antiserum treatment on E12 significantly attenuated receptor mRNA and protein levels by P8, but equine chorionic gonadotropin replacement on P1 reversed the inhibition. Furthermore, FSH in vitro up-regulated BMPR levels in P4 ovaries. This unique pattern of BMPR expression in the oocytes and somatic cells during perinatal ovary development suggests that BMP may play a regulatory role in primordial follicle formation. Furthermore, FSH may regulate BMP action by modulating the expression of its receptors. (Endocrinology 150: 1886-1896, 2009)