Reduced levels of histone H3 acetylation on the inactive X chromosome in human females
Reduced levels of histone H3 acetylation on the inactive X chromosome in human females
复制标题
DOI：
10.1007/s004120050188
复制
发表时间：
1996-12-01
期刊：
影响因子：
1.6
通讯作者：
Allis, CD
中科院分区：
文献类型：
--
作者：
Boggs, BA;Connors, B;Allis, CD
产生的新型抗体对四膜虫和人类等多种生物体中的组蛋白 H3 乙酰化或非乙酰化异构体或组蛋白 H4 的乙酰化形式具有高度选择性。在免疫细胞学分析中使用这些抗体作为成对组，我们证明雌性哺乳动物细胞中组蛋白 H3 和 H4 的失活 X 染色体均被低乙酰化，而识别组蛋白 H3 非乙酰化形式的抗体可以统一识别所有染色体。这些数据验证并扩展了先前的结果，并表明核心组蛋白的低乙酰化可能是沿着失活 X 染色体的染色质的一般特征。
Novel antibodies were generated that are highly selective for either acetylated or unacetylated isoforms of histone H3, or the acetylated form of histone H4 in organisms as diverse as Tetrahymena and humans. Using these antibodies as pair-wise sets in immunocytological analyses, we demonstrate that the inactive X chromosome is hypoacetylated for both histone H3 and H4 in female mammalian cells, whereas the antibody that recognizes the unacetylated form of histone H3 identifies all chromosomes uniformly. These data verify and extend previous results and suggest that hypoacetylation of core histones may be a general feature of the chromatin along the inactive X chromosome.