Colorimetric assay based on peroxidase-like activity of dodecyl trimethylammonium bromide-tetramethyl zinc (4-pyridinyl) porphyrin for detection of organophosphorus pesticides
Colorimetric assay based on peroxidase-like activity of dodecyl trimethylammonium bromide-tetramethyl zinc (4-pyridinyl) porphyrin for detection of organophosphorus pesticides
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DOI：
10.1007/s00604-022-05430-2
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发表时间：
2022-09
影响因子：
5.7
通讯作者：
Gaoqiong Deng;Hengye Chen;Qiong Shi;Lixue Ren;Ke Liang;Wanjun Long;Wei Lan;Xiaole Han;Yuanbin She;Haiyan Fu
中科院分区：
文献类型：
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作者：
Gaoqiong Deng;Hengye Chen;Qiong Shi;Lixue Ren;Ke Liang;Wanjun Long;Wei Lan;Xiaole Han;Yuanbin She;Haiyan Fu
以3,3′，5,5′-四甲基联苯胺(TMB)/过氧化氢(H2O2)/十二烷基三甲基溴化铵(DTAB)-四甲基锌（4-吡啶基）卟啉（ZnTPyP）为检测试剂，建立了一种简便、灵敏的有机磷农药（OPs）比色法。在该体系中，基于DTAB-ZnTPyP的过氧化物酶样活性，h2o2分解产生羟基自由基，羟基自由基氧化TMB，生成蓝色氧化产物。OPs（敌百虫、敌敌畏和硫胺）首先通过静电相互作用与DTAB-ZnTPyP结合。OPs导致DTAB-ZnTPyP的过氧化物酶样活性因空间位点阻断而降低。同时，它们之间发生π-相互作用，这些相互作用也抑制了TMB （652nm）的氧化，从而使OPs的检测成为可能。敌百虫、敌敌畏和硫磷的检出限分别为0.25、1.02和0.66 μg/L，线性范围分别为1 ~ 35、5 ~ 45和1 ~ 40 μg/L。该方法可用于测定白菜、苹果、土壤和中药样品中的OPs（回收率为91.8 ~ 109.8%），在其他复杂样品中也具有很好的应用前景。
A simple and sensitive colorimetric assay for detecting organophosphorus pesticides (OPs) was developed based on3,3',5,5'-tetramethylbenzidine (TMB)/hydrogen peroxide (H2O2)/dodecyl trimethylammonium bromide (DTAB)-tetramethyl zinc (4-pyridinyl) porphyrin (ZnTPyP). In this system, based on the peroxidase-like activity of DTAB-ZnTPyP, H2O2decomposes to produce hydroxyl radicals, which oxidize TMB, resulting in blue oxidation products. The OPs (trichlorfon, dichlorvos, and thimet) were first combined with DTAB-ZnTPyP through electrostatic interactions. The OPs caused a decrease in the peroxidase-like activity of DTAB-ZnTPyP due to spatial site blocking. At the same time, π-interactions occurred between them, and these interactions also inhibited the oxidation of TMB (652 nm), thus making the detection of OPs possible. The limits of detection for trichlorfon, dichlorvos, and thimet were 0.25, 1.02, and 0.66 μg/L, respectively, and the corresponding linear ranges were 1–35, 5–45, and 1–40 μg/L, respectively. Moreover, the assay was successfully used to determine OPs in cabbage, apple, soil, and traditional Chinese medicine samples (the recovery ratios were 91.8–109.8%), showing a great promising potential for detecting OPs also in other complex samples.