Dynamic Light Scattering Based Microelectrophoresis: Main Prospects and Limitations.
Dynamic Light Scattering Based Microelectrophoresis: Main Prospects and Limitations.
复制标题
DOI：
10.1080/01932691.2011.625523
复制
发表时间：
2012-12-01
影响因子：
2.2
通讯作者：
Uskoković V
中科院分区：
文献类型：
--
作者：
Uskoković V
基于动态光散射（DLS）效应的微电泳技术已成为评价和控制胶体体系稳定性条件的主要工具。然而，用于表征分散的亚微米颗粒的流体动力学尺寸的DLS方法和电动现象背后的理论都与限制其灵敏度和信息输出的基本和实际近似相关联。其中一些基本的限制，包括DLS测量的球形近似和胶体系统的微电泳分析无法检测离散电荷和不同的带电粒子表面之间由于旋转扩散和粒子取向平均，在这项工作中重新审视。沿着，对这两种分析方法的主要发展前景进行了展望。对zeta电位在生物化学过程中的作用也作了详细的综述。虽然zeta电位已被用作控制胶体分散体稳定性的主要参数之一，但它的应用前景更为广阔。操纵相互作用物种的表面电荷在设计复杂的软物质形态的概念，最近深入研究，作为一个例子。DLS和zeta电位分析是胶体化学领域的一个分支，现在越来越多地应用于药物输送、生物技术、纳米现象的物理化学和其他站在当代科学前沿的研究领域。耦合基于DLS的微电泳系统与互补的表征方法被提到作为增加这两种分析技术的信息输出的繁荣的路径之一。
Microelectrophoresis based on the dynamic light scattering (DLS) effect has been a major tool for assessing and controlling the conditions for stability of colloidal systems. However, both the DLS methods for characterization of the hydrodynamic size of dispersed submicron particles and the theory behind the electrokinetic phenomena are associated with fundamental and practical approximations that limit their sensitivity and information output. Some of these fundamental limitations, including the spherical approximation of DLS measurements and an inability of microelectrophoretic analyses of colloidal systems to detect discrete charges and differ between differently charged particle surfaces due to rotational diffusion and particle orientation averaging, are revisited in this work. Along with that, the main prospects of these two analytical methods are mentioned. A detailed review of the role of zeta potential in processes of biochemical nature is given too. It is argued that although zeta potential has been used as one of the main parameters in controlling the stability of colloidal dispersions, its application potentials are much broader. Manipulating surface charges of interacting species in designing complex soft matter morphologies using the concept of zeta potential, intensively investigated recently, is given as one of the examples. Branching out from the field of colloid chemistry, DLS and zeta potential analyses are now increasingly finding application in drug delivery, biotechnologies, physical chemistry of nanoscale phenomena and other research fields that stand on the frontier of the contemporary science. Coupling the DLS-based microelectrophoretic systems with complementary characterization methods is mentioned as one of the prosperous paths for increasing the information output of these two analytical techniques.
登录
查看更多内容
影响因子：
3.3
作者：
Andreozzi, Patrizia;Funari, Sergio S.;Spinozzi, Francesco
通讯作者：
Spinozzi, Francesco
影响因子：
9.9
作者：
Adamczyk, Z.;Nattich, M.;Sadowska, M.
通讯作者：
Sadowska, M.
影响因子：
3
作者：
Aichmayer, B;Margolis, HC;Fratzl, P
通讯作者：
Fratzl, P
DOI：
10.1023/a:1021177601899
发表时间：
2002-12-01
影响因子：
3.7
作者：
Botelho, CM;Lopes, MA;Santos, JD
通讯作者：
Santos, JD
影响因子：
3.3
作者：
Adamczyk, Z.;Bratek, A.;Warszynski, P.
通讯作者：
Warszynski, P.