STRUCTURE OF A COLLAGEN-GAG DERMAL SKIN SUBSTITUTE OPTIMIZED FOR CULTURED HUMAN EPIDERMAL-KERATINOCYTES
STRUCTURE OF A COLLAGEN-GAG DERMAL SKIN SUBSTITUTE OPTIMIZED FOR CULTURED HUMAN EPIDERMAL-KERATINOCYTES
复制标题
DOI：
10.1002/jbm.820221008
复制
发表时间：
1988-10-01
期刊：
影响因子：
--
通讯作者：
HANSBROUGH, JF
中科院分区：
文献类型：
--
作者：
BOYCE, ST;CHRISTIANSON, DJ;HANSBROUGH, JF
研究了胶原和糖胺聚糖 (GAG) 真皮替代品（膜）作为培养人表皮角质形成细胞的基质。真皮替代品的结构针对孔径进行了优化，以促进纤维血管组织从伤口床向内生长，并用于培养膜表面的人类角质形成细胞。通过将冷冻温度控制在-50℃至-20℃之间以及通过将起始材料的浓度控制在0.17℃之间来调节冻干材料的孔径。和 1.62% 重量/体积。将胶原蛋白-GAG 的无孔表面层压到膜上，为培养的表皮角质形成细胞提供平面基质。通过控制起始材料的体积和浓度来调节真皮替代物的厚度。生物素与溶解的胶原蛋白缀合，用于与特定数量的生物活性分子的抗生物素蛋白结合。优化后的膜是培养人表皮角质形成细胞的合适基质，并与细胞一起产生组织学上与皮肤相似的复合材料。
Collagen and glycosaminoglycan (GAG) dermal skin substitutes (membranes) were studied as substrates for cultured human epidermal keratinocytes. Structure of dermal substitutes was optimized for pore size to promote ingrowth of fibrovascular tissue from the wound bed and for culture of human keratinocytes of the membrane''s surface. Pore size of the freeze-dried material was regulated by control of the temperature of freezing between -50.degree.C and -20.degree.C and by concentration of starting materials between 0.17.degree. and 1.62% wt/vol. A nonporous surface of collagen-GAG was laminated to the membranes to provide a planar substrate for cultured epidermal keratinocytes. Thickness of dermal substitutes was regulated by control of the volume and concentration of starting materials. Biotin was conjugated to solubilized collagen for binding with avidin of specific quantities of biologically active molecules. The optimized membranes are suitable substrates for the culture of human epidermal keratinocytes, and together with the cells yield a composite material that is histologically similar to skin.