Developmental expression of alcohol dehydrogenase (ADH3) in zebrafish (Danio rerio)
Developmental expression of alcohol dehydrogenase (ADH3) in zebrafish (Danio rerio)
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DOI：
10.1006/bbrc.2001.5511
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发表时间：
2001-09-07
影响因子：
3.1
通讯作者：
Carvan, MJ
中科院分区：
文献类型：
--
作者：
Dasmahapatra, AK;Doucet, HL;Carvan, MJ
乙醇脱氢酶（ADH）是负责将乙醇代谢为乙醛的主要酶。已经在鱼中描述了一类ADH，并且已经发现其在结构上类似于哺乳动物III类ADH（谷胱甘肽依赖性甲醛脱氢酶），但是。功能上类似于I类ADH（主要负责乙醇代谢）。我们通过RT-PCR从斑马鱼（Danio rerio）肝脏中克隆了一个代表斑马鱼ADH 3基因产物的cDNA，编码区为1131个核苷酸。推导出的氨基酸序列与海水鱼类Sparesaurata的ADH 3的同源性为90%，与小鼠和人的同源性分别为82%和81%。采用定量竞争RT-PCR方法。ADH 3的mRNA检测在所有的时间点分析，是最低的受精后8和24小时之间。因此，ADH 3的差异表达可能是至少部分负责斑马鱼胚胎发育酒精暴露的敏感性的时间变化。(C)北京：科学出版社.
Alcohol dehydrogenase (ADH) is the primary enzyme responsible for metabolism of ethanol to acetaldehyde. One class of ADH has been described in fish, and has been found to be structurally similar to mammalian class III ADH (glutathione-dependent formaldehyde dehydrogenase) but. functionally similar to class I ADH (primarily responsible for ethanol metabolism). We have cloned a cDNA by RT-PCR from zebrafish (Danio rerio) liver representing the zebrafish ADH3 gene product, with a coding region of 1131 nucleotides. The deduced amino acid sequences share 90% identity to ADH3 from the marine fish Spares aurata, and 82 and 81%a identity to the mouse and human sequences, respectively. Using a quantitative competitive RT-PCR assay. ADH3 mRNA was detected at all timepoints analyzed and was, lowest between 8 and 24 h postfertilization. Thus, differential ADH3 expression may be at least partly responsible for temporal variations in the sensitivity of zebrafish embryos to developmental alcohol exposure. (C) 2001 Academic Press.