Maleness-on-the-Y (MoY) orthologue is a key regulator of male sex determination in Zeugodacus cucurbitae (Diptera: Tephritidae)
Maleness-on-the-Y (MoY) orthologue is a key regulator of male sex determination in Zeugodacus cucurbitae (Diptera: Tephritidae)
复制标题
Maleness-on-the-Y (MoY) 直系同源物是 Zeugodacus cucurbitae（双翅目：实蝇科）雄性性别决定的关键调节因子
DOI：
10.1016/j.jia.2022.08.007
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发表时间：
2023-01-26
影响因子：
4.8
通讯作者：
Lin Xian-wu
中科院分区：
文献类型：
--
作者：
Fan Zi-zhen;Ma Qin;Lin Xian-wu
昆虫性别分化的启动受初级性别决定信号的调控。在水蝇、头型角毛虫和其他头翅目动物中，雄性在Y上(Moy)是决定雄性性别的主要基因。然而，瓜实蝇(Zeugoacus cucurbitae(Coquillett))的主要信号仍然不明确。在本研究中，我们分离并鉴定了南瓜ZcMoY中的MedFly Moy同源物。ZcMOY蛋白在细菌中与其同源物具有高度的序列保守性。ZcMoY转录在非常早期的胚胎阶段开始并达到顶峰，然后除了第一天的雄性成体的睾丸和头部外，变得无法检测到。通过RNAi沉默早期胚胎中的ZcMoY会导致外生殖器和内部生殖器官的异常，导致XY个体的中间性和女性化。ZcMoY的表达模式和敲除表型表明，ZcMoY在调节葫芦科雄性个体的性别决定中起着关键作用。我们的研究结果将有助于了解葫芦科植物性别决定的机制，并有助于基因性别鉴定品系在其生物防治中的发展。
The initiation of sex differentiation in insects is regulated by primary sex determination signals. In the Medfly Ceratitis capitata and other tephritids, Maleness-on-the-Y (MoY) is the master gene for male sex determination. However, the primary signal in Zeugodacus cucurbitae (Coquillett), a very destructive tephritid pest across the world, remains ambiguous. In this study, we have isolated and characterized the Medfly MoY homolog in Z. cucurbitae, ZcMoY. ZcMOY protein shows high sequence conservation to its homologs in Bactrocera species. ZcMoY transcription begins and peaks at very early embryonic stages and then becomes undetectable except the testes and heads of day 1 male adults. Silencing ZcMoY in early embryos by RNAi causes abnormal external genitalia and interior reproductive organs, giving rise to intersexes and feminization of XY individuals. The expression pattern and knockdown phenotypes of ZcMoY indicate that ZcMoY plays a key role in regulating sex determination of Z. cucurbitae males. Our findings will help the understanding of sex determination in Z. cucurbitae and facilitate the development of genetic sexing strains in its biological control.