Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis
Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis
复制标题
适配体固定的开放管状毛细管柱捕获循环肿瘤细胞用于蛋白质组分析
DOI：
10.1016/j.talanta.2017.07.041
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发表时间：
2017
期刊：
影响因子：
6.1
通讯作者：
Zhang Yukui
中科院分区：
文献类型：
--
作者：
Liu Lukuan;Yang Kaiguang;Zhu Xudong;Liang Yu;Chen Yuanbo;Fang Fei;Zhao Qun;Zhang Lihua;Zhang Yukui
Circulating tumor cells hold the key to predicting the prognosis and discovering the therapeutic targets. Herein, we proposed a strategy to develop an aptamer-immobilized open tubular capillary column by which SMMC-7721 human hepatoma cells (SMMC-7721 cells) could be captured with an over 70% of capture efficiency and a 3.0 ± 0.2 of enrichment factor. Owing to the compatibility of the column, the captured cells by the column could be analyzed by LC-MS from protein level and 5 unique proteins of SMMC-7721 cells were identified which could be used as markers to identify SMMC-7721 cells when Jurkat T-leukemia cells (Jurkat cells) were employed as interfering cells. As the key component, the aptamer-immobilized column had the potential to be integrated into the platform for separating, enriching and characterizing rare cells simultaneously.
